Quantitative analysis of the heteromeric neuronal nicotinic receptors in the rat hippocampus.
Quantitative analysis of the heteromeric neuronal nicotinic receptors in the rat hippocampus.
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DOI：
10.1111/j.1471-4159.2010.06967.x
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发表时间：
2010-11
影响因子：
4.7
通讯作者：
Kellar KJ
中科院分区：
文献类型：
--
作者：
Lomazzo E;MacArthur L;Yasuda RP;Wolfe BB;Kellar KJ
The objective of this study was to identify and quantify the heteromeric neuronal nicotinic receptors (nAChRs) in the rat hippocampus. The density of nAChR subtypes was assessed by labeling them with [3H]epibatidine followed by immunoprecipitation with subunit-selective antibodies. Sequential immunoprecipitation assays were used to establish associations between two different subunits, which then allowed the full subunit composition of the receptors to be deduced. Our results show that most of the hippocampal heteromeric nAChRs contain α4 and β2 subunits. In fact, we identified two populations containing these two predominant subunits, the α4β2 and α4β2α5 subtypes which account for ~40% and ~35%, respectively, of the total [3H]epibatidine-labeled receptors. An additional heteromeric subtype with the subunit composition of α4β2α3 represented ~10% of the total nAChRs, and another 10% of the immunoprecipitated receptors contained α4 and β4 subunits, with or without the α3 subunit. To determine if α4β2 and α4β2α5 nAChR subtypes differ in their ligand binding affinities, the α3- and β4-containing receptors were first removed by immunoprecipitation and then, competition studies with acetylcholine, nicotine, cytisine and sazetidine-A against [3H]epibatidine were carried out on the remaining α4β2 and α4β2α5 subtypes. Results suggested these subtypes have comparable binding affinities for the nicotinic ligands used here.
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影响因子：
16.2
作者：
CONROY, WG;VERNALLIS, AB;BERG, DK
通讯作者：
BERG, DK
影响因子：
3.4
作者：
Jia, Yousheng;Yamazaki, Yoshihiko;Sumikawa, Katumi
通讯作者：
Sumikawa, Katumi
影响因子：
3.6
作者：
Mao, Danyan;Yasuda, Robert P.;Kellar, Kenneth J.
通讯作者：
Kellar, Kenneth J.
影响因子：
7.3
作者：
Clarke, PBS;Reuben, M
通讯作者：
Reuben, M
DOI：
10.1523/jneurosci.3952-08.2009
发表时间：
2009-01-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Liu Q;Huang Y;Xue F;Simard A;DeChon J;Li G;Zhang J;Lucero L;Wang M;Sierks M;Hu G;Chang Y;Lukas RJ;Wu J
通讯作者：
Wu J