Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin.
Development of a high-throughput assay to detect antibody inhibition of low pH induced conformational changes of influenza virus hemagglutinin.
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DOI：
10.1371/journal.pone.0199683
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Li ZN
中科院分区：
文献类型：
--
作者：
Trost JF;LeMasters EH;Liu F;Carney P;Lu X;Sugawara K;Hongo S;Stevens J;Steinhauer DA;Tumpey T;Katz JM;Levine MZ;Li ZN
Many broadly neutralizing antibodies (bnAbs) bind to conserved areas of the hemagglutinin (HA) stalk region and can inhibit the low pH induced HA conformational changes necessary for viral membrane fusion activity. We developed and evaluated a high-throughput virus-free and cell-free ELISA based low pH induced HA Conformational Change Inhibition Antibody Detection Assay (HCCIA) and a complementary proteinase susceptibility assay. Human serum samples (n = 150) were tested by HCCIA using H3 recombinant HA. Optical density (OD) ratios of mAb HC31 at pH 4.8 to pH 7.0 ranged from 0.87 to 0.09. Our results demonstrated that low pH induced HA conformational change inhibition antibodies (CCI) neutralized multiple H3 strains after removal of head-binding antibodies. The results suggest that HCCIA can be utilized to detect and characterize CCI in sera, that are potentially broadly neutralizing, and serves as a useful tool for evaluating universal vaccine candidates targeting the HA stalk.
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影响因子：
3.8
作者：
DANIELS, RS;DOUGLAS, AR;WILEY, DC
通讯作者：
WILEY, DC
影响因子：
5.4
作者：
Eggink, Dirk;Goff, Peter H.;Palese, Peter
通讯作者：
Palese, Peter
影响因子：
7.6
作者：
Laursen, Nick S.;Wilson, Ian A.
通讯作者：
Wilson, Ian A.
中和和非中和人类H7N9流感疫苗诱导的单克隆抗体提供保护。
影响因子：
30.3
作者：
Henry Dunand CJ;Leon PE;Huang M;Choi A;Chromikova V;Ho IY;Tan GS;Cruz J;Hirsh A;Zheng NY;Mullarkey CE;Ennis FA;Terajima M;Treanor JJ;Topham DJ;Subbarao K;Palese P;Krammer F;Wilson PC
通讯作者：
Wilson PC
DOI：
10.1126/science.1204839
发表时间：
2011-08-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ekiert DC;Friesen RH;Bhabha G;Kwaks T;Jongeneelen M;Yu W;Ophorst C;Cox F;Korse HJ;Brandenburg B;Vogels R;Brakenhoff JP;Kompier R;Koldijk MH;Cornelissen LA;Poon LL;Peiris M;Koudstaal W;Wilson IA;Goudsmit J
通讯作者：
Goudsmit J