Quantitative Trait Locus Mapping of Resistance to Turnip Yellows Virus in Brassica rapa and Brassica oleracea and Introgression of These Resistances by Resynthesis Into Allotetraploid Plants for Deployment in Brassica napus.
Quantitative Trait Locus Mapping of Resistance to Turnip Yellows Virus in Brassica rapa and Brassica oleracea and Introgression of These Resistances by Resynthesis Into Allotetraploid Plants for Deployment in Brassica napus.
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DOI：
10.3389/fpls.2021.781385
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发表时间：
2021
影响因子：
5.6
通讯作者：
Walsh JA
中科院分区：
文献类型：
--
作者：
Greer SF;Hackenberg D;Gegas V;Mitrousia G;Edwards D;Batley J;Teakle GR;Barker GC;Walsh JA
芜菁黄病毒（TuYV）是一种蚜虫传播的病毒，在油菜（OSR，芸苔，基因组：AACC）和蔬菜芸苔中造成相当大的产量损失。由于杀虫剂抗药性和欧盟禁止使用最有效的活性成分，对蚜虫媒介的杀虫剂控制受到限制。在目前的商业OSR品种中，TuYV抗性只有一个来源，已定位为A04染色体上的一个显性数量性状位点（QTL）。本研究报道了油菜（OSR）二倍体祖种、油菜（Brassica rapa，基因组：AA）和甘蓝（Brassica oleracea，基因组：CC）对TuYV抗性的鉴定、表征和定位。抗性和易感个体之间的种内杂交产生的F1群体的表型分析表明，抗性是定量的和部分显性的。分离回交群体的QTL定位表明，rapa抗性至少由两个加性QTL控制，分别位于A02染色体和A06染色体上。他们共同解释了40.3%的表型变异。在甘蓝中，C05染色体上的单个QTL解释了22.1%的表型变异。本研究检测到的TuYV抗性qtl与现有的商业抗性品种不同。为了利用这些抗性，利用抗tuyv的油菜和甘蓝种间杂交，重新合成了一个异源四倍体（基因组：AACC）植物品系。流式细胞术证实，由种间杂交再生的植株具有A和C两个基因组，为混合倍体。为了稳定倍性，一株可育植株进行自花授粉，产生的种子具有所需的再合成异源四倍体基因组AACC。重组植株的表型分析证实了它们对TuYV的抗性。利用在祖细胞图谱中鉴定的抗性连锁标记进行基因分型，证实了来自rapa和甘蓝的所有TuYV抗性qtl的存在。这是首次在芸苔C基因组中定位到对TuYV的抗性，以及一个同源四倍体AACC系对TuYV具有双重抗性的报道。利用标记辅助选择，现在可以加速向OSR的渗入，这可能会减少能够克服现有TuYV抗性来源的TuYV分离株的选择压力。
Turnip yellows virus (TuYV) is aphid-transmitted and causes considerable yield losses in oilseed rape (OSR, Brassica napus, genome: AACC) and vegetable brassicas. Insecticide control of the aphid vector is limited due to insecticide resistance and the banning of the most effective active ingredients in the EU. There is only one source of TuYV resistance in current commercial OSR varieties, which has been mapped to a single dominant quantitative trait locus (QTL) on chromosome A04. We report the identification, characterisation, and mapping of TuYV resistance in the diploid progenitor species of OSR, Brassica rapa (genome: AA), and Brassica oleracea (genome: CC). Phenotyping of F1 populations, produced from within-species crosses between resistant and susceptible individuals, revealed the resistances were quantitative and partially dominant. QTL mapping of segregating backcross populations showed that the B. rapa resistance was controlled by at least two additive QTLs, one on chromosome A02 and the other on chromosome A06. Together, they explained 40.3% of the phenotypic variation. In B. oleracea, a single QTL on chromosome C05 explained 22.1% of the phenotypic variation. The TuYV resistance QTLs detected in this study are different from those in the extant commercial resistant varieties. To exploit these resistances, an allotetraploid (genome: AACC) plant line was resynthesised from the interspecific cross between the TuYV-resistant B. rapa and B. oleracea lines. Flow cytometry confirmed that plantlets regenerated from the interspecific cross had both A and C genomes and were mixoploid. To stabilise ploidy, a fertile plantlet was self-pollinated to produce seed that had the desired resynthesised, allotetraploid genome AACC. Phenotyping of the resynthesised plants confirmed their resistance to TuYV. Genotyping with resistance-linked markers identified during the mapping in the progenitors confirmed the presence of all TuYV resistance QTLs from B. rapa and B. oleracea. This is the first report of TuYV resistance mapped in the Brassica C genome and of an allotetraploid AACC line possessing dual resistance to TuYV originating from both of its progenitors. The introgression into OSR can now be accelerated, utilising marker-assisted selection, and this may reduce selection pressure for TuYV isolates that are able to overcome existing sources of resistance to TuYV.
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影响因子：
3.8
作者：
HALEY, CS;KNOTT, SA
通讯作者：
KNOTT, SA
影响因子：
5
作者：
Congdon, B. S.;Baulch, J. R.;Coutts, B. A.
通讯作者：
Coutts, B. A.
影响因子：
1.2
作者：
He Z;Cheng F;Li Y;Wang X;Parkin IA;Chalhoub B;Liu S;Bancroft I
通讯作者：
Bancroft I
影响因子：
3.2
作者：
Hunter, PJ;Jones, JE;Walsh, JA
通讯作者：
Walsh, JA
影响因子：
2
作者：
Diederichsen, E;Sacristan, MD
通讯作者：
Sacristan, MD