KITENIN promotes glioma invasiveness and progression, associated with the induction of EMT and stemness markers.
KITENIN promotes glioma invasiveness and progression, associated with the induction of EMT and stemness markers.
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DOI：
10.18632/oncotarget.3087
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发表时间：
2015-02-20
期刊：
影响因子：
--
通讯作者：
Moon KS
中科院分区：
文献类型：
--
作者：
Lee KH;Ahn EJ;Oh SJ;Kim O;Joo YE;Bae JA;Yoon S;Ryu HH;Jung S;Kim KK;Lee JH;Moon KS
KAI 1 COOH-末端相互作用四跨膜蛋白（KAI 1 COOH-terminal interacting tetraspanin）促进多种癌症的肿瘤侵袭和转移。本研究评估了患者中KITENIN表达与晚期胶质瘤分级之间的相关性。体外实验表明，KITENIN敲低抑制胶质瘤细胞的侵袭和迁移，而KITENIN过表达促进胶质瘤细胞的侵袭和迁移。在原位小鼠肿瘤模型中，移植有KITENIN转染的神经胶质瘤细胞的小鼠的存活期显著短于移植有模拟转染细胞的小鼠。与高表达的患者相比，低表达的患者显示出显著更长的无进展生存期。KITENIN诱导上皮-间质转化（EMT）标志物（N-钙粘蛋白、ZEB 1、ZEB 2、SNAIL和SLUG）以及胶质瘤干性标志物（CD 133、ALDH 1和EPH-B1）的表达。总之，这些发现表明高水平的KITENIN增加胶质瘤的侵袭性和进展，与EMT和干性标志物的上调相关。
KITENIN (KAI1 COOH-terminal interacting tetraspanin) promotes tumor invasion and metastasis in various cancers. This study assessed the association between KITENIN expression and advanced glioma grade in patients. In vitro assays revealed that KITENIN knockdown inhibited the invasion and migration of glioma cells, whereas KITENIN overexpression promoted their invasion and migration. In orthotopic mouse tumor models, mice transplanted with KITENIN-transfected glioma cells had significantly shorter survival than mice transplanted with mock-transfected cells. Patients with low KITENIN expression showed a significantly longer progression-free survival than patients with high KITENIN expression. KITENIN induced the expression of the epithelial-mesenchymal transition (EMT) markers (N-cadherin, ZEB1, ZEB2, SNAIL and SLUG) as well as the glioma stemness markers (CD133, ALDH1 and EPH-B1). Taken together, these findings showed that high levels of KITENIN increased glioma invasiveness and progression, associated with the up-regulation of EMT and stemness markers.
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影响因子：
6
作者：
Huang, CI;Kohno, N;Miyake, M
通讯作者：
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