Myc regulates programmed cell death and radial glia dedifferentiation after neural injury in an echinoderm.
Myc regulates programmed cell death and radial glia dedifferentiation after neural injury in an echinoderm.
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DOI：
10.1186/s12861-015-0071-z
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发表时间：
2015-05-30
影响因子：
--
通讯作者：
García-Arrarás JE
中科院分区：
文献类型：
--
作者：
Mashanov VS;Zueva OR;García-Arrarás JE
成年棘皮动物即使在严重受伤后，也能完全再生其中枢神经系统的主要部分。尽管这一能力早已为人所知，但推动这些动物快速和完全再生的分子机制仍未得到研究。理解这些分子途径的主要障碍是缺乏关于再生的成年棘皮动物的功能基因组研究。在这里，我们使用RNA干扰介导的基因敲除来表征Myc在海参海参神经损伤后早期(第一个48小时)反应中的作用。我们先前的实验发现，Myc是唯一的多能相关因子，其在损伤的CNS中的表达显著增加。然而，这个基因的具体功能(S)仍然未知。在这里，我们证明了Myc的敲除抑制了放射状胶质细胞的去分化和细胞的程序性死亡，这两个最显著的细胞事件发生在损伤后不久再生的海参神经系统中。在这项研究中，我们证明了Myc的过度表达是放射状胶质细胞适当去分化和触发损伤附近的程序性细胞死亡所必需的。因此，MYC是第一个转录因子，其在棘皮再生中的作用已被实验确定。本文的在线版本(doi：10.1186/s12861-0150071-z)包含补充材料，授权用户可以使用。
Adult echinoderms can completely regenerate major parts of their central nervous system even after severe injuries. Even though this capacity has long been known, the molecular mechanisms that drive fast and complete regeneration in these animals have remained uninvestigated. The major obstacle for understanding these molecular pathways has been the lack of functional genomic studies on regenerating adult echinoderms. Here, we employ RNA interference-mediated gene knockdown to characterize the role of Myc during the early (first 48 hours) post-injury response in the radial nerve cord of the sea cucumber Holothuria glaberrima. Our previous experiments identified Myc as the only pluripotency-associated factor, whose expression significantly increased in the wounded CNS. The specific function(s) of this gene, however, remained unknown. Here we demonstrate that knockdown of Myc inhibits dedifferentiation of radial glia and programmed cell death, the two most prominent cellular events that take place in the regenerating sea cucumber nervous system shortly after injury. In this study, we show that Myc overexpression is required for proper dedifferentiation of radial glial cells and for triggering the programmed cell death in the vicinity of the injury. Myc is thus the first transcription factor, whose functional role has been experimentally established in echinoderm regeneration. The online version of this article (doi:10.1186/s12861-015-0071-z) contains supplementary material, which is available to authorized users.
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转录组中枢神经系统在棘皮动物中的再生过程中的变化。
影响因子：
4.4
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