Conservation and regulation of alternative splicing by dynamic inter- and intra-intron base pairings in Lepidoptera 14-3-3z pre-mRNAs
Conservation and regulation of alternative splicing by dynamic inter- and intra-intron base pairings in Lepidoptera 14-3-3z pre-mRNAs
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鳞翅目 14-3-3z 前体 mRNA 中动态内含子间和内含子碱基配对对选择性剪接的保护和调节
作者：
Jin Y;Sun F;Wang X;Yue Y;Wang W;Zhang W;Zhan L;Tian N;Shi F
前体mRNA的选择性剪接极大地促进了由单个基因产生的产物的多样性。然而，人们对潜在的调控机制知之甚少。在本研究中，我们描述了进化上保守的内含子内和内含子间RNA二级结构。突变实验表明，内含子内RNA二级结构导致空间位阻以执行相互剪接排他性，而内含子间RNA配对主要通过环出机制起作用。此外，相互排斥的剪接可以通过内含子内和内含子间的RNA配对竞争来调节。重要的是，产生的动态RNA结构在很大程度上控制互斥剪接，尽管顺式作用调控序列可以微调这一过程。我们的研究结果强调了动态RNA结构在选择性剪接中的重要性。
Alternative splicing of pre-mRNAs greatly contributes to diversity in products generated from a single gene. However, the underlying regulatory mechanisms are poorly understood. In the present study, we describe evolutionarily conserved intra-intronic and inter-intronic RNA secondary structures. Mutation experiments revealed that intra-intronic RNA secondary structure causes steric hindrance to enforce mutual splicing exclusivity, while inter-intronic RNA pairing largely functions through a looped-out mechanism. Moreover, mutually exclusive splicing may be regulated by RNA pairing competition between intra- and inter-introns. Importantly, the resulting dynamic RNA architecture largely controls mutually exclusive splicing, although cis-acting regulatory sequences may fine-tune this process. Our results emphasize the importance of dynamic RNA architecture in alternative splicing.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.5
作者：
Lee, Christopher;Kim, Namshin;Graveley, Brenton R.
通讯作者：
Graveley, Brenton R.
DOI：
--
发表时间：
2003
期刊：
The EMBO journal
影响因子：
--
作者：
N. Gromak;Alexis P Rideau;J. Southby;A. Scadden;C. Gooding;S. Hüttelmaier;R. Singer;Christopher W. J. Smith
通讯作者：
N. Gromak;Alexis P Rideau;J. Southby;A. Scadden;C. Gooding;S. Hüttelmaier;R. Singer;Christopher W. J. Smith
影响因子：
4.8
作者：
Muh, SJ;Hovhannisyan, RH;Carstens, RP
通讯作者：
Carstens, RP
影响因子：
64.5
作者：
Graveley, BR
通讯作者：
Graveley, BR