Deciphering postnatal limb development at single-cell resolution.
Deciphering postnatal limb development at single-cell resolution.
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以单细胞分辨率解读出生后肢体发育
DOI：
10.1016/j.isci.2022.105808
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发表时间：
2023-01-20
期刊：
影响因子：
5.8
通讯作者：
Zhou, Zhiyu
中科院分区：
文献类型：
--
作者：
Gao, Manman;Liu, Xizhe;Guo, Peng;Wang, Jianmin;Li, Junhong;Wang, Wentao;Stoddart, Martin J.;Grad, Sibylle;Li, Zhen;Wu, Huachuan;Li, Baoliang;He, Zhongyuan;Zhou, Guangqian;Liu, Shaoyu;Zhu, Weimin;Chen, Dafu;Zou, Xuenong;Zhou, Zhiyu
出生后早期的肢体发育过程是胚胎和成熟阶段的桥梁，反映了关节软骨的病理性重塑。然而，与众多关于胚胎肢体发育的研究相比，出生后早期似乎相对不受关注。在这里，通过使用单细胞RNA测序技术表征来自小鼠后肢的19，952个出生后4个阶段的单细胞，进行了描绘出生后肢体发育景观的系统工作。通过细胞异质性的描绘，在关节软骨和附着点中发现了标记为Cd 34和Ly6e的候选祖细胞亚群，并且在生长板中反映了标记为Col10a1、Spp 1和Tnni 2的三个细胞发育分支。本文对骨关节炎中具有代表性的转录组和发育模式进行了深入研究，并对骨关节炎的关键调控机制和演变进行了讨论。最重要的是，这些结果扩展了出生后肢体发育生物学的视野，并达到肢体发育，重塑和再生之间的相互联系。出生后肢体发育景观的描绘在关节软骨和附着点中发现候选祖细胞在出生后生长板中的三个细胞发育分支的解析达到出生后肢体发育和病理重塑的互连生物科学;组学;转录组学
The early postnatal limb developmental progression bridges embryonic and mature stages and mirrors the pathological remodeling of articular cartilage. However, compared with multitudinous research on embryonic limb development, the early postnatal stage seems relatively unnoticed. Here, a systematic work to portray the postnatal limb developmental landscape was carried out by characterization of 19,952 single cells from murine hindlimbs at 4 postnatal stages using single-cell RNA sequencing technique. By delineation of cell heterogeneity, the candidate progenitor sub-clusters marked by Cd34 and Ly6e were discovered in articular cartilage and enthesis, and three cellular developmental branches marked by Col10a1, Spp1, and Tnni2 were reflected in growth plate. The representative transcriptomes and developmental patterns were intensively explored, and the key regulation mechanisms as well as evolvement in osteoarthritis were discussed. Above all, these results expand horizons of postnatal limb developmental biology and reach the interconnections between limb development, remodeling, and regeneration. Delineation of postnatal limb developmental landscape Discovery of candidate progenitors in articular cartilage and enthesis Parsing of three cellular developmental branches in postnatal growth plate Reaching interconnections of postnatal limb development and pathological remodeling Biological sciences; Omics; Transcriptomics
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