The Quorum Sensing System of Yersinia enterocolitica 8081 Regulates Swimming Motility, Host Cell Attachment, and Virulence Plasmid Maintenance.
The Quorum Sensing System of Yersinia enterocolitica 8081 Regulates Swimming Motility, Host Cell Attachment, and Virulence Plasmid Maintenance.
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DOI：
10.3390/genes9060307
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发表时间：
2018-06-20
期刊：
影响因子：
3.5
通讯作者：
Atkinson S
中科院分区：
文献类型：
--
作者：
Ng YK;Grasso M;Wright V;Garcia V;Williams P;Atkinson S
尽管小肠结肠炎耶尔森氏菌基因组具有高度异质性，但它们包含保守的 N-酰基高丝氨酸内酯依赖性 (AHL) 群体感应 (QS) 系统，分别由 luxR 和 luxI 直向同源物 YanR 和 YenI 组成。某些高毒力菌株还含有假定的孤儿 luxR 基因 ycoR，该基因与 AHL 合酶无关。为了探讨小肠结肠炎耶尔森菌菌株 8081 中 YenR/yenI/ycoR 对 QS 依赖性表型的贡献，构建了单个和多个突变体。 AHL 分析确定 N-(3-氧代己酰基) 高丝氨酸内酯、N-己酰基高丝氨酸内酯和 N-(3-氧代庚酰基) 高丝氨酸内酯最为丰富。日元R、ycoR和yenR/ycoR突变体的AHL谱与亲本相似，表明这两个LuxR同源物不调节AHL产生，而yenI突变体是AHL阴性的。 QS 在游泳运动和细胞附着中的作用得到了证实。在yenI和yenI/yenR/ycoR突变体中毒力质粒分区基因spyA的下调与yenI突变体中小肠结肠炎耶尔森氏菌pYVe毒力质粒在37℃连续传代过程中的更大损失一致，但在22℃下则不然。提出了 QS 调节的间谍 A 在毒力质粒维持中的作用。
Although Yersinia enterocolitica genomes are highly heterogeneous, they contain a conserved N-acylhomoserine lactone-dependent (AHL) quorum sensing (QS) system consisting of the luxR and luxI orthologs yenR and yenI respectively. Certain hypervirulent strains also contain a putative orphan luxR gene, ycoR, that is not linked to an AHL synthase. To explore the contribution of yenR/yenI/ycoR to QS-dependent phenotypes in Yersinia enterocolitica strain 8081, single and multiple mutants were constructed. AHL profiling identified N-(3-oxohexanoyl) homoserine lactone, N-hexanoylhomoserine lactone, and N-(3-oxoseptanoyl) homoserine lactone as the most abundant. The AHL profiles of the yenR, ycoR and yenR/ycoR mutants were similar to the parent suggesting that the two LuxR homologues do not regulate AHL production while the yenI mutants were AHL-negative. A role for QS in swimming motility and cell attachment was demonstrated. Down-regulation of the virulence plasmid partition gene, spyA, in yenI and yenI/yenR/ycoR mutants is consistent with the greater loss of the Y. enterocolitica pYVe virulence plasmid in the yenI mutant during serial passage at 37 °C but not at 22 °C. A role for QS-regulated spyA in virulence plasmid maintenance is suggested.
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影响因子：
6.7
作者：
Atkinson S;Goldstone RJ;Joshua GW;Chang CY;Patrick HL;Cámara M;Wren BW;Williams P
通讯作者：
Williams P
DOI：
10.1099/00221287-143-12-3703
发表时间：
1997-12-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
McClean, KH;Winson, MK;Williams, P
通讯作者：
Williams, P
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.2
作者：
Atkinson, S;Chang, CY;Williams, P
通讯作者：
Williams, P
影响因子：
5.7
作者：
Dewoody RS;Merritt PM;Marketon MM
通讯作者：
Marketon MM