Regulation of the Yersinia type III secretion system: traffic control.
Regulation of the Yersinia type III secretion system: traffic control.
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DOI：
10.3389/fcimb.2013.00004
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发表时间：
2013
影响因子：
5.7
通讯作者：
Marketon MM
中科院分区：
文献类型：
--
作者：
Dewoody RS;Merritt PM;Marketon MM
耶尔森氏菌和许多其他革兰氏阴性病原体一样，使用III型分泌系统(T3SS)将效应蛋白从细菌细胞质转移到宿主细胞质。这种T3SS类似于一个分子注射器，有一个针状的杆子连接到一个基本的身体结构，它横跨细菌的内外膜。注射体的基体与细菌鞭毛高度同源。从T3SS基底体延伸出来的是针，它是一种单一蛋白质YscF的聚合物。针的远端用作组装由LcrV组成的尖端复合体的平台。虽然从未直接观察到，但流行的模型假设LcrV有助于将形成孔的蛋白YopB和YopD插入宿主细胞膜。这完成了细菌和宿主细胞之间的桥梁，提供了一个连续的通道，通过这个通道传递效应剂。为了了解T3SS是如何组装的，它的底物是如何识别的，以及底物输送是如何控制的，已经做出了大量的努力。可以说，后一个主题是最不被理解的；然而，最近的进展提供了新的见解，因此，本综述将主要集中在总结关于T3SS控制底物递送的知识现状。具体地说，我们将讨论YopK以及YopN和YopE的作用，它们长期以来一直与易位调控联系在一起。我们还提出了YopK调节器与T3SS的基本体通信以控制易位的模型。
Yersinia species, as well as many other Gram-negative pathogens, use a type III secretion system (T3SS) to translocate effector proteins from the bacterial cytoplasm to the host cytosol. This T3SS resembles a molecular syringe, with a needle-like shaft connected to a basal body structure, which spans the inner and outer bacterial membranes. The basal body of the injectisome shares a high degree of homology with the bacterial flagellum. Extending from the T3SS basal body is the needle, which is a polymer of a single protein, YscF. The distal end of the needle serves as a platform for the assembly of a tip complex composed of LcrV. Though never directly observed, prevailing models assume that LcrV assists in the insertion of the pore-forming proteins YopB and YopD into the host cell membrane. This completes a bridge between the bacterium and host cell to provide a continuous channel through which effectors are delivered. Significant effort has gone into understanding how the T3SS is assembled, how its substrates are recognized and how substrate delivery is controlled. Arguably the latter topic is the least understood; however, recent advances have provided new insight, and therefore, this review will focus primarily on summarizing the current state of knowledge regarding the control of substrate delivery by the T3SS. Specifically, we will discuss the roles of YopK, as well as YopN and YopE, which have long been linked to regulation of translocation. We also propose models whereby the YopK regulator communicates with the basal body of the T3SS to control translocation.
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影响因子：
3.6
作者：
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通讯作者：
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3.6
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