A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria.
A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria.
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DOI：
10.1002/anie.201802796
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发表时间：
2018-07-16
期刊：
影响因子：
--
通讯作者：
Kelley SO
中科院分区：
文献类型：
--
作者：
Jiménez-Sánchez A;Lei EK;Kelley SO
测量活细胞中的理化参数可以提供特定于单个细胞器的信息，帮助我们了解健康和疾病中的亚细胞功能。虽然细胞器特异性化学探针已经允许定性测量细胞器如线粒体中的微极性或微粘度，但线粒体局部微粘度（ηm）和微极性（εm）沿着结构并发结构变化的同时定量仍然是未满足的需求。在这里，我们描述了一种新的多功能线粒体探针（MMP）的同时监测ηm和εm。这种新的探针能够在各种干扰细胞功能的试剂的存在下高度精确地定量测量局部线粒体微粘度和微极性，并且使用探针观察到的变化也可以与线粒体网络形态和运动性的改变相关。所描述的策略是一个很有前途的工具，用于细胞器结构的细微变化的分析。一种新的荧光探针能够在各种干扰细胞功能的试剂的存在下高度精确地定量测量局部线粒体微粘度和微极性，其中观察到的变化也可能与线粒体网络形态和运动性的改变相关。
The measurement of physicochemical parameters in living cells can provide information specific to individual cell organelles, helping us to understand subcellular function in health and disease. While organelle-specific chemical probes have allowed qualitative measurements of micropolarity or microviscosity in organelles such as the mitochondria, the simultaneous quantification of mitochondrial local microviscosity (ηm) and micropolarity (εm), along with structural concurrent structural variations, has remained an unmet need. Here, we describe a new multifunctional mitochondrial probe (MMP) for simultaneous monitoring of ηm and εm. This new probe enabled highly precise, quantitative measurements of local mitochondrial microviscosity and micropolarity in the presence of a variety of agents perturbing cellular function, and the changes observed using the probe could also be correlated with alterations in mitochondrial network morphology and motility. The strategy described represents a promising tool for the analysis of subtle changes in organellar structure. A new fluorescent probe enables highly precise, quantitative measurements of local mitochondrial microviscosity and micropolarity in the presence of a variety of agents perturbing cellular function, where the observed changes could also be correlated with alterations in mitochondrial network morphology and motility.
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影响因子：
16.1
作者：
Wen R;Banik B;Pathak RK;Kumar A;Kolishetti N;Dhar S
通讯作者：
Dhar S
影响因子：
15
作者：
Dodani, Sheel C.;Leary, Scot C.;Cobine, Paul A.;Winge, Dennis R.;Chang, Christopher J.
通讯作者：
Chang, Christopher J.
影响因子：
3.5
作者：
Salvioli, S;Ardizzoni, A;Cossarizza, A
通讯作者：
Cossarizza, A
DOI：
10.1073/pnas.1310583110
发表时间：
2014-01-07
影响因子：
11.1
作者：
Chyan, Wen;Zhang, Daniel Y.;Radford, Robert J.
通讯作者：
Radford, Robert J.
影响因子：
4
作者：
Park, Jong-Seok;Nam, Eunju;Rhee, Hyun-Woo
通讯作者：
Rhee, Hyun-Woo