Morpholino monolayers: preparation and label-free DNA analysis by surface hybridization.
Morpholino monolayers: preparation and label-free DNA analysis by surface hybridization.
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DOI：
10.1021/ja810051q
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发表时间：
2009-04-08
影响因子：
15
通讯作者：
Levicky R
中科院分区：
文献类型：
--
作者：
Tercero N;Wang K;Gong P;Levicky R
Surface hybridization, a reaction in which nucleic acid molecules in solution react with nucleic acid partners immobilized on a surface, is widely practiced in life science research. In these applications the immobilized partner, or "probe", is typically single-stranded DNA. Because DNA is strongly charged, high salt conditions are required to enable binding between analyte nucleic acids ("targets") in solution and the DNA probes. High salt, however, compromises prospects for label-free monitoring or control of the hybridization reaction through surface electric fields, as well as stabilizes secondary structure in target species that can interfere with probe-target recognition. In this work, initial steps toward addressing these challenges are taken by introducing Morpholinos, a class of uncharged DNA analogues, for surface-hybridization applications. Monolayers of Morpholino probes on gold supports can be fabricated with methods similar to those employed with DNA, and are shown to hybridize efficiently and sequence-specifically with target strands. Hybridization-induced changes in the interfacial charge organization are analyzed with electrochemical methods and compared for Morpholino and DNA probe monolayers. Molecular mechanisms connecting surface hybridization state to the interfacial capacitance are identified and interpreted through comparison to numerical Poisson Boltzmann calculations. Interestingly, positive as well as negative capacitive responses (contrast inversion) to hybridization are possible, depending on surface populations of mobile ions as controlled by the applied potential. Quantitative comparison of surface capacitance with target coverage (targets/area) reveals a near-linear relationship, and demonstrates sensitivities (limits of quantification) in the pg mm−2 range.
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影响因子：
12.6
作者：
Ghindilis, Andrey L.;Smith, Maria W.;McShea, Andy
通讯作者：
McShea, Andy
DOI：
10.1016/0006-291x(66)90447-5
发表时间：
1966-01-01
影响因子：
3.1
作者：
DENHARDT, DT
通讯作者：
DENHARDT, DT
影响因子：
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作者：
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通讯作者：
Jacob, A
影响因子：
4.3
作者：
Guiducci, Carlotta;Stagni, Claudio;Ricco, Bruno
通讯作者：
Ricco, Bruno
影响因子：
2.9
作者：
Glazer, Marc;Fidanza, Jacqueline A.;Frank, Curts W.
通讯作者：
Frank, Curts W.