Multi-laboratory analysis of the variability of shipped samples for proteomics following non-cooled international transport.
Multi-laboratory analysis of the variability of shipped samples for proteomics following non-cooled international transport.
复制标题
对非冷藏国际运输后蛋白质组学运输样品的变异性进行多实验室分析
DOI：
10.1016/j.ab.2018.02.026
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发表时间：
2018
影响因子：
2.9
通讯作者：
Schlüter H
中科院分区：
文献类型：
--
作者：
Steffen P;Krisp C;Yang P;Molloy MP;Schlüter H
由于需要保持完整性并最大限度地减少修饰和降解，运输细胞或组织等生物样品变得复杂，但这在经济上成本高昂，因为样品必须在冷冻状态下运输。这项多实验室研究调查了使用质谱法进行蛋白质组分析时干燥肽样品的非冷却运输所带来的样品变异性。人类癌细胞胰蛋白酶裂解物在澳大利亚进行蛋白水解和干燥，然后空运到欧洲和中国。在所有三个地点的类似 LC-MS 系统上使用无标记质谱法测量样品。以这种方式制备和分析样本在蛋白质鉴定方面仅产生微小差异，并且在参与实验室中显示出较高的定量再现性。我们检查了对肽化学修饰的任何影响，并报告与起始、未运输的样品相比没有差异。我们的结论是，非冷却干燥肽的运输对下游 LC-MS 分析的样品完整性的影响可以忽略不计，因此是促进国际蛋白质组学合作的一种经济有效的选择。数据可通过 ProteomeXchange 获取，标识符为 PXD008160。
Transporting biological samples such as cells or tissues is complicated by the need to maintain integrity and minimise modification and degradation, but this is economically costly as the samples must be shipped in a frozen state. This multi-laboratory study investigated sample variability introduced by non-cooled transport of dried peptide samples for proteomic analysis using mass spectrometry. Human cancer cell tryptic lysates were proteolysed and dried in Australia and shipped by air to Europe and China. Samples were measured using label free mass spectrometry on similar LC-MS systems at all three sites. Preparation and analysis of the specimens in this manner resulted in only minor differences in protein identification and showed high quantitative reproducibility amongst the participating laboratories. We examined any impact on peptide chemical modification and report no discrepancies compared to the starting, non-shipped sample. We conclude that transport of non-cooled, dried peptides has negligible effect on sample integrity for downstream LC-MS analysis and therefore represents a cost-effective option to facilitate international proteomic collaborations.Data is available via ProteomeXchange with identifier PXD008160.
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影响因子：
4.4
作者：
Tabb, David L.;Vega-Montoto, Lorenzo;Rudnick, Paul A.;Variyath, Asokan Mulayath;Ham, Amy-Joan L.;Bunk, David M.;Kilpatrick, Lisa E.;Billheimer, Dean D.;Blackman, Ronald K.;Cardasis, Helene L.;Carr, Steven A.;Clauser, Karl R.;Jaffe, Jacob D.;Kowalski, Kevin A.;Neubert, Thomas A.;Regnier, Fred E.;Schilling, Birgit;Tegeler, Tony J.;Wang, Mu;Wang, Pei;Whiteaker, Jeffrey R.;Zimmerman, Lisa J.;Fisher, Susan J.;Gibson, Bradford W.;Kinsinger, Christopher R.;Mesri, Mehdi;Rodriguez, Henry;Stein, Stephen E.;Tempst, Paul;Paulovich, Amanda G.;Liebler, Daniel C.;Spiegelman, Cliff
通讯作者：
Spiegelman, Cliff
对仪器平台和实验工作流程进行实验室间评估，以进行定量准确性和再现性评估
影响因子：
--
作者：
A. Percy;Jessica Tamura;J. Albar;K. Aloria;A. Amirkhani;Gabriel D. T. Araujo;J. M. Arizmendi;F. Blanco;F. Canals;Jin‐Young Cho;Núria Colomé;F. Corrales;G. Domont;Guadalupe Espadas;P. Fernández‐Puente;C. Gil;P. Haynes;M. Hernáez;Jin;A. Kopylov;Miguel Marcilla;Mathew J. McKay;M. Mirzaei;M. Molloy;Leanne B. Ohlund;Y. Paik;A. Paradela;M. Raftery;E. Sabidó;L. Sleno;Daniel Wilffert;J. Wolters;J. Yoo;V. Zgoda;C. Parker;C. Borchers
通讯作者：
C. Borchers
影响因子：
7.4
作者：
Liu, HB;Sadygov, RG;Yates, JR
通讯作者：
Yates, JR
影响因子：
3.8
作者：
Dick, Lawrence W., Jr.;Kim, Catherine;Cheng, Kuang-Chuan
通讯作者：
Cheng, Kuang-Chuan