Wood smoke particle exposure in mice reduces the severity of influenza infection.
Wood smoke particle exposure in mice reduces the severity of influenza infection.
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小鼠暴露于木材烟雾颗粒可降低流感感染的严重程度。
DOI：
10.1016/j.taap.2021.115645
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发表时间：
2021-09-01
影响因子：
3.8
通讯作者：
Tighe RM
中科院分区：
文献类型：
--
作者：
Vose A;McCravy M;Birukova A;Yang Z;Hollingsworth JW;Que LG;Tighe RM
环境温度升高和极端天气事件增加了世界范围内野火的发生率，导致木材烟雾颗粒（WSP）增加。流行病学数据表明，WSP暴露与呼吸道疾病的恶化和呼吸道病毒感染的增加有关。为了评估WSP暴露对宿主对病毒性肺炎反应的影响，我们在啮齿动物中进行了WSP暴露，然后感染小鼠适应性流感（HINI-PR 8）。在急性（单剂量）或亚急性暴露（第1、3、5、7和10天）中，通过口咽抽吸用WSP或PBS攻击6-8周龄的C57 BL/6雄性小鼠。其他组接受亚急性暴露，然后感染流感病毒或热灭活（HI）病毒。暴露/感染后，进行支气管肺泡灌洗（BAL）以评估总细胞计数/分类、总蛋白、蛋白羰基和透明质酸。通过真实的时间PCR评估肺组织的病毒计数。当与PBS相比时，急性WSP暴露与空域巨噬细胞的增加相关。或者，亚急性暴露导致空域中性粒细胞呈剂量依赖性增加。流感感染后的亚急性WSP暴露与呼吸道病毒结局改善相关，包括体重减轻和血氧饱和度增加，以及蛋白质羰基和病毒滴度降低。流式细胞术显示基于WSP和流感病毒攻击的肺巨噬细胞和T细胞亚群的动态变化。该数据表明，亚急性WSP暴露可以改善宿主对急性流感感染的反应。
Elevated ambient temperatures and extreme weather events have increased the incidence of wildfires world-wide resulting in increased wood smoke particle (WSP). Epidemiologic data suggests that WSP exposure associates with exacerbations of respiratory diseases, and with increased respiratory viral infections. To assess the impact of WSP exposure on host response to viral pneumonia, we performed WSP exposures in rodents followed by infection with mouse adapted influenza (HINI-PR8). C57BL/6 male mice aged 6–8 weeks were challenged with WSP or PBS by oropharyngeal aspiration in acute (single dose) or sub-acute exposures (day 1, 3, 5, 7 and 10). Additional groups underwent sub-acute exposure followed by infection by influenza or heat-inactivated (HI) virus. Following exposures/infection, bronchoalveolar lavage (BAL) was performed to assess for total cell counts/differentials, total protein, protein carbonyls and hyaluronan. Lung tissue was assessed for viral counts by real time PCR. When compared to PBS, acute WSP exposure associated with an increase in airspace macrophages. Alternatively, sub-acute exposure resulted in a dose dependent increase in airspace neutrophils. Sub-acute WSP exposure followed by influenza infection was associated with improved respiratory viral outcomes including reduced weight loss and increased blood oxygen saturation, and decreased protein carbonyls and viral titers. Flow cytometry demonstrated dynamic changes in pulmonary macrophage and T cell subsets based on challenge with WSP and influenza. This data suggests that sub-acute WSP exposure can improve host response to acute influenza infection.
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影响因子：
10.2
作者：
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通讯作者：
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DOI：
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