Activation of Na:2Cl:K cotransport by luminal chloride in macula densa cells.
Activation of Na:2Cl:K cotransport by luminal chloride in macula densa cells.
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致密斑细胞中鲁米那氯化物激活 Na:2Cl:K 共转运。
DOI：
10.1038/ki.1995.115
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发表时间：
1995
影响因子：
19.6
通讯作者：
Bell,PD
中科院分区：
文献类型：
--
作者：
Lapointe,JY;Laamarti,A;Hurst,AM;Fowler,BC;Bell,PD
Activation of Na:2Cl:K cotransport by luminal chloride in macula densa cells. Changes in macula densa intracellular pH (pHi) were used to monitor the direction of flux mediated by the apical Na:2Cl:K cotransporter. At the macula densa, a decrease in luminal [Cl] ([Cl]1) from 60 to 1 mMproduced cellular alkalinization secondary to a cascade of events involving a decrease in apical Na:2Cl:K cotransport, a fall in intracellular [Na] ([Na]i) and a stimulation of Na:H exchange. This is supported by the fact that 97% of the change in macula densa pHiwith reduction in [Cl]1was bumetanide-sensitive whereas 92% of this pH change was amiloride-sensitive. We found that, in the presence of 20 mMNa and 5 mMK, a [Cl]1of 14.3 ± 2.4 mM(N = 7) produced equilibrium of the apical cotransporter since the pHiobtained under this condition was identical to the pHifound after reducing the net ionic flux to zero with bumetanide. Using this value together with the expected stoichiometry for the bumetanide-sensitive cotransporter, it was estimated that the intracellular [Cl] ([Cl]1) at equilibrium (or in the presence of bumetanide) could be as low as 5 mM. Also, using a Hill number of 2 which is consistent with the present data, the affinity for [Cl]1was found to be 32.5 mM. Under physiological luminal conditions prevailing at the end of the thick ascending limb (-3.5 mMK, and -25 to 30 mMNaCl), macula densa cells are probably operating close to equilibrium while maintaining a small net reabsorption of Na/K and Cl. Since macula densa cells appear capable of reducing [Cl]ito very low levels, a reabsorptive flux should continue to occur until [NaCl]1is reduced to 18 mM.
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DOI：
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DOI：
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