Identification and Tracking of Alloreactive T Cell Clones in Rhesus Macaques Through the RM-scTCR-Seq Platform.
Identification and Tracking of Alloreactive T Cell Clones in Rhesus Macaques Through the RM-scTCR-Seq Platform.
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DOI：
10.3389/fimmu.2021.804932
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发表时间：
2021
影响因子：
7.3
通讯作者：
Kean LS
中科院分区：
文献类型：
--
作者：
Gerdemann U;Fleming RA;Kaminski J;McGuckin C;Rui X;Lane JF;Keskula P;Cagnin L;Shalek AK;Tkachev V;Kean LS
T细胞受体（TCR）克隆型追踪是用于询问T细胞介导的免疫过程的有力工具。将单个细胞的转录程序与其TCR身份配对的新方法允许监测T细胞克隆型特异性转录动力学。虽然这些技术已用于人类和小鼠T细胞研究，但尚未开发用于恒河猴（RM），这是一种用于自身免疫性疾病，疫苗开发和移植的关键翻译生物。我们描述了一种新的管道“RM-scTCR-Seq”，它首次实现了RM特异性单细胞TCR的扩增、重建以及RM TCR与其转录谱的配对。我们将该方法应用于GVHD的RM模型，并在GVHD靶器官中识别和追踪体外检测到的同种异体反应性克隆型，并探索其GVHD驱动的细胞毒性T细胞特征。这种新颖的、最先进的平台从根本上推进了RM在研究保护性和致病性T细胞应答方面的应用。
T cell receptor (TCR) clonotype tracking is a powerful tool for interrogating T cell mediated immune processes. New methods to pair a single cell’s transcriptional program with its TCR identity allow monitoring of T cell clonotype-specific transcriptional dynamics. While these technologies have been available for human and mouse T cells studies, they have not been developed for Rhesus Macaques (RM), a critical translational organism for autoimmune diseases, vaccine development and transplantation. We describe a new pipeline, ‘RM-scTCR-Seq’, which, for the first time, enables RM specific single cell TCR amplification, reconstruction and pairing of RM TCR’s with their transcriptional profiles. We apply this method to a RM model of GVHD, and identify and track in vitro detected alloreactive clonotypes in GVHD target organs and explore their GVHD driven cytotoxic T cell signature. This novel, state-of-the-art platform fundamentally advances the utility of RM to study protective and pathogenic T cell responses.
高通量测序综合分析恒河猴T细胞受体β链基因
影响因子：
4.6
作者：
Li Z;Liu G;Tong Y;Zhang M;Xu Y;Qin L;Wang Z;Chen X;He J
通讯作者：
He J
DOI：
10.1111/j.1600-6143.2011.03737.x
发表时间：
2012-01
期刊：
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
影响因子：
--
作者：
Page A;Srinivasan S;Singh K;Russell M;Hamby K;Deane T;Sen S;Stempora L;Leopardi F;Price AA;Strobert E;Reimann KA;Kirk AD;Larsen CP;Kean LS
通讯作者：
Kean LS