Relationships between energy level and insulin secretion in isolated rat islets of Langerhans. A study at various pH values.
Relationships between energy level and insulin secretion in isolated rat islets of Langerhans. A study at various pH values.
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离体大鼠朗格汉斯岛能量水平与胰岛素分泌之间的关系。
DOI：
10.1016/0006-2952(91)90322-v
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发表时间：
1991
影响因子：
5.8
通讯作者：
Erecińska,M
中科院分区：
文献类型：
--
作者：
Ohta,M;Nelson,D;Nelson,J;Meglasson,MD;Erecińska,M
为了更好地确定[三磷酸腺苷]/[腺苷二磷酸]在胰岛胰岛素释放中的作用，我们将外部pH变化引起的腺嘌呤核苷酸比率的变化与两种代谢性分泌剂-16 mM葡萄糖和10 mMα-异己酸产生的分泌谱相关联。实验是在pH为6.5-7.7的有无重碳酸盐的缓冲液中进行的。胰岛素的释放依赖于pHe1，与使用的促分泌剂无关。α-酮基异己酸在有无碳酸氢盐的情况下分泌情况相同；释放量降至pH 7.1以下，但保持在7.4-7.7。在用HEPES缓冲的培养液中，葡萄糖也出现了同样的情况。在碳酸氢盐存在的情况下，高糖引起的分泌物对[H+]呈钟形依赖，在pH 7.1-7.4的酸性和碱性侧减少。在所研究的所有pH值下，以HEPES为缓冲液时，[ATP]和[ADP]均较高。在基础和刺激条件下，[ATP]/[ADP]均随pH升高而降低，刺激后[ATP]/[ADP]值始终较大，但在pH为7.7时，以碳酸氢钠为底物，以葡萄糖为刺激剂时，差异很小。结果表明：(1)胰岛[ATP]/[ADP]明显依赖于Phe；(2)[ATP]或[ATP]/[ADP]绝对值与胰岛素分泌无直接相关性；(3)[ATP]/[ADP]升高是葡萄糖刺激的胰岛素释放所必需的，但并非总是速率决定事件。
To define better the role of [ATP]/[ADP] in insulin release from pancreatic islets, changes in the adenine nucleotide ratios elicited by alterations in external pH were correlated with the secretion profiles produced by administration of two metabolic secretagogues, 16 mM glucose and 10 mM α-ketoisocaproic acid. Experiments were carried out in buffers with and without bicarbonate, in the pH range 6.5–7.7. Insulin release was dependent on pHeirrespective of the secretagogue used. Secretion profiles for α-ketoisocaproic acid were the same both with and without bicarbonate; the release was decreased below pH 7.1 but maintained at 7.4–7.7. The same pattern was seen with glucose in media buffered with Hepes. With bicarbonate present, secretion caused by high glucose showed a bell-shaped dependence on [H+], with reductions at the acid and alkaline sides of pH 7.1–7.4. [ATP] and [ADP] were higher when Hepes was the buffer, at all pH values studied. The [ATP]/[ADP] declined with increasing pH under both basal and stimulated conditions; the values were always larger after stimulation although at pH 7.7 with bicarbonate present and glucose as the stimulant the difference was very small. It is concluded that: (i) the [ATP]/[ADP] in pancreatic islets is markedly dependent on pHe; (ii) there is no straight-forward correlation between either [ATP] or the absolute value for [ATP]/[ADP] and insulin secretion; and (iii) a rise in [ATP]/[ADP] is necessary for glucose-stimulated insulin release although it is not always the rate-determining event.
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通过荧光比率显微镜评估单个胰腺 β 细胞的 pH 调节。
DOI：
--
发表时间：
1989
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
E. Grapengiesser;E. Gylfe;B. Hellman
通讯作者：
B. Hellman
磷酸盐的变化不会影响离体小鼠朗格汉斯岛的胰岛素释放。
DOI：
--
发表时间：
1988
期刊：
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
影响因子：
--
作者：
B. Frankel;S. Sjunghall
通讯作者：
S. Sjunghall
缺磷对碳水化合物代谢的影响*
DOI：
--
发表时间：
1978
期刊：
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
影响因子：
--
作者：
W. Marshall;M. Banasiak;R. Kalkhoff
通讯作者：
R. Kalkhoff
影响因子：
11.2
作者：
R. Mccomb;W. D. Yushok
通讯作者：
W. D. Yushok
影响因子：
2.9
作者：
D. W. Schulz;J. Passonneau;O. H. Lowry
通讯作者：
O. H. Lowry