Expression of Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Their Receptors (Flt-1, Flk-1, and Flg-1) in Canine Vascular Tumors
Expression of Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Their Receptors (Flt-1, Flk-1, and Flg-1) in Canine Vascular Tumors
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血管内皮生长因子、碱性成纤维细胞生长因子及其受体（Flt-1、Flk-1和Flg-1）在犬血管肿瘤中的表达
DOI：
10.1354/vp.43-6-971
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发表时间：
2006
影响因子：
2.4
通讯作者：
T. Masegi
中科院分区：
文献类型：
--
作者：
K. Yonemaru;H. Sakai;M. Murakami;T. Yanai;T. Masegi
采用免疫组化方法研究犬血管肉瘤（HSA）和血管瘤组织中血管内皮生长因子（VEGF）、受体flt-1和flk-1、碱性成纤维细胞生长因子（bFGF）的表达。此外，通过逆转录聚合酶链式反应（RT-PCR）和原位杂交（ISH）与cRNA探针分析VEGF、flt-1、flk-1和flg-1 （bFGF的受体）mrna的表达。免疫组化法检测HSAs肿瘤细胞中VEGF、bFGF、flt-1、flk-1的表达；HSAs的染色强度高于血管瘤。另一方面，在血管瘤的肿瘤细胞中，尽管flt-1和flk-1有中等程度的表达，但VEGF的表达非常弱或不表达。采用ISH和RT-PCR检测HSAs肿瘤细胞中VEGF、flt-1、flk-1、flg-1 mrna的表达。而在血管瘤的肿瘤细胞中，ISH检测不到VEGF mRNA，而在肿瘤的炎症细胞中，RT-PCR检测到VEGF mRNA。此外，flk-1染色强烈的HSAs具有较高的增殖活性，表现为较高的Ki-67阳性指数。提示生长因子及其受体的表达，特别是flk-1的表达可能与HSAs的恶性增殖有关。
Expression of vascular endothelial growth factor (VEGF), its receptors (flt-1 and flk-1), and basic fibroblast growth factor (bFGF) in canine hemangiosarcoma (HSA) and hemangiomas was investigated by immunohistochemical analysis. In addition, expression of the mRNAs of VEGF, flt-1, flk-1, and flg-1 (a receptor for bFGF), was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR) and in situ hybridization (ISH) with cRNA probes. VEGF, bFGF, flt-1, and flk-1 were immunohistochemically detected in the neoplastic cells in HSAs; the staining intensity was stronger in HSAs than in hemangiomas. On the other hand, the neoplastic cells in hemangiomas exhibited very weak or no expression of VEGF, although they showed moderate expression of flt-1 and flk-1. The mRNAs of VEGF, flt-1, flk-1, and flg-1 were detected in the neoplastic cells in HSAs by ISH and RT-PCR. However, VEGF mRNA was not detectable in the neoplastic cells in hemangiomas by ISH, although it was detected in the inflammatory cells in the tumors by RT-PCR. Moreover, the HSAs that showed intense staining for flk-1 had a high proliferative activity, which was reflected as a high Ki-67 positive index. These results suggest that the expression of the growth factors and their receptors, especially flk-1, might be associated with the malignant proliferation of HSAs.
影响因子：
5.2
作者：
D. Bielenberg;C. Bucana;R. Sanchez;J. Mulliken;J. Folkman;I. Fidler
通讯作者：
D. Bielenberg;C. Bucana;R. Sanchez;J. Mulliken;J. Folkman;I. Fidler
影响因子：
4.8
作者：
Akhtar, N;Padilla, ML;Helfand, SC
通讯作者：
Helfand, SC