UV irradiation causes multiple cellular changes in cultured human retinal pigment epithelium cells
UV irradiation causes multiple cellular changes in cultured human retinal pigment epithelium cells
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紫外线照射引起培养的人视网膜色素上皮细胞的多种细胞变化
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
S. Thanos
中科院分区：
文献类型：
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作者：
K. Tratsk;S. Thanos
背景视网膜色素上皮可能参与了年龄相关性黄斑变性的发生和发展，但其发生机制尚不清楚。本研究的目的是检查细胞的变化，在视网膜色素上皮细胞诱导的直接照射与紫外线culture.MethodsRetinal色素上皮细胞从死后的人视网膜被用来获得解离培养细胞保留的能力，在体外分化。将这些细胞培养数天至数周。UV辐射（UV-A和UV-B）在无菌条件下发生，其中100 HBO/水银灯泡连接到解剖显微镜，提供同轴照明。辐射效应的时间依赖性进行了分析，使用形态计量学，免疫组化，功能和automotosis-detecting techniques. ResultsVitality和增殖的视网膜色素上皮细胞培养物可以一致地制备。细胞在体外表现出组织特异性形态数天至数周。色素上皮衍生因子检测在这些细胞中使用免疫细胞化学和Western印迹。UV照射而非白色光导致细胞形状和大小的可测量的改变。照射后的细胞表现出部分肿胀和收缩，使人联想到进行性凋亡变性。TUNEL染色显示紫外光可诱导细胞凋亡，而白色光不能诱导细胞凋亡。荧光微粒的吞噬作用在照射后减弱。这些影响依赖于照射的持续时间。ConclusionsCultures的视网膜色素上皮细胞是合适的和敏感的模型来研究细胞损伤，并可能有助于解开视网膜变性的发病机制。
BackgroundThe retinal pigment epithelium maybe causally involved in the development and progression of age-related macula degeneration; however, the mechanisms leading to the development of age-related macula degeneration remain largely unknown. The purpose of this study was to examine cellular changes in the retinal pigment epithelium induced by direct irradiation with UV light in culture.MethodsRetinal pigment epithelium cells from post-mortem human retinas were used to obtain dissociated cultures with cells retaining the ability to differentiate in vitro. These cells were cultured over several days to weeks. The UV radiation (UV-A and UV-B) occurred under sterile conditions with a 100 HBO/mercury bulb attached to a dissecting microscope, delivering co-axial illumination. The time dependence of irradiation effects was analysed using morphometric, immunohistochemical, functional and apoptosis-detecting techniques.ResultsVital and proliferating retinal pigment epithelium cell cultures could be prepared consistently. The cells showed tissue-specific morphologies in vitro for several days to weeks. Pigment epithelium-derived factor was detected in these cells using immunocytochemistry and Western blots. The UV irradiation but not white light resulted in measurable alterations of cell shape and size. The irradiated cells showed partial swelling and shrinkage reminiscent of progressing apoptotic degeneration. TUNEL staining revealed that apoptosis was induced by UV light, but not detectably by white light. The phagocytosis of fluorescent micro-particles diminished after irradiation. These effects were dependent on the duration of irradiation.ConclusionsCultures of retinal pigment epithelium are suitable and sensitive models to study cell damage and may contribute to unravelling the pathogenetic mechanisms of retinal degeneration.
影响因子：
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作者：
B. Klein;R. Klein
通讯作者：
B. Klein;R. Klein
影响因子：
2.2
作者：
P. Karakousis;Sinoj K. John;K. Behling;E. Surace;Julie E. Smith;A. Hendrickson;W. Tang;J. Bennett-J.-Benn
通讯作者：
P. Karakousis;Sinoj K. John;K. Behling;E. Surace;Julie E. Smith;A. Hendrickson;W. Tang;J. Bennett-J.-Benn