Designing nanomaterial-enhanced electrochemical immunosensors for cancer biomarker proteins.
Designing nanomaterial-enhanced electrochemical immunosensors for cancer biomarker proteins.
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DOI：
10.1016/j.bioelechem.2009.03.011
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发表时间：
2009-09
影响因子：
5
通讯作者：
Papadimitrakopoulosa, Fotios
中科院分区：
文献类型：
--
作者：
Rusling, James F.;Sotzing, Gregory;Papadimitrakopoulosa, Fotios
在护理点检测人血清和组织中的多种癌症生物标志物蛋白是早期癌症检测的可行方法，但对生物分析设备开发提出了重大挑战。本文综述了我们实验室最近开发的结合纳米颗粒修饰电极和多标记二抗标记颗粒以实现高灵敏度检测癌症生物标志物蛋白的方法。使用了两种基于纳米材料的传感器平台：（a）直立单壁碳纳米管森林和（B）密集堆积的5 nm金纳米颗粒层。这两种平台都具有悬垂的羧酸酯基团，便于通过酰胺化连接酶或抗体。在质量性能测试中，用于测定过氧化氢的AuNP层与辣根过氧化物酶（HRP）的电极上的生物催化反应给出了更好的检测限和灵敏度比单壁碳纳米管（SWNT）森林平台与HRP连接。对这些传感器作为血清中癌症生物标志物前列腺特异性抗原（PSA）的夹心免疫测定平台的评估表明，这两种方法对来自癌症患者的人血清样品都给出了准确的结果。最佳检测限（0.5 pg mL−1）和灵敏度通过将AuNP免疫传感器与用二抗和7500 HRP标记修饰的1 μm直径磁性颗粒结合来获得。
Detection of multiple cancer biomarker proteins in human serum and tissue at point-of-care is a viable approach for early cancer detection, but presents a major challenge to bioanalytical device development. This article reviews recent approaches developed in our laboratories combining nanoparticle decorated electrodes and multi-labeled secondary antibody labeled particles to achieve high sensitivity for the detection of cancer biomarker proteins. Two nanomaterials-based sensor platforms were used: (a) upright single wall carbon nanotube forests and (b) layers of densely packed 5 nm gold nanoparticles. Both platforms feature pendant carboxylate groups for easy attachment of enzymes or antibodies by amidization. In quality performance tests, the biocatalytic responses for determination of hydrogen peroxide of AuNP layers with attached horseradish peroxidase (HRP) on electrodes gave somewhat better detection limit and sensitivity than single wall carbon nanotube (SWNT) forest platforms with HRP attached. Evaluation of these sensors as platforms for sandwich immunoassays for cancer biomarker prostate specific antigen (PSA) in serum showed that both approaches gave accurate results for human serum samples from cancer patients. The best detection limit (0.5 pg mL−1) and sensitivity were obtained by combining the AuNP immunosensors with binding of 1 μm diameter magnetic particles decorated with secondary antibodies and 7500 HRP labels.
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影响因子：
17.1
作者：
Mani, Vigneshwaran;Chikkaveeraiah, Bhaskara V.;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
7.4
作者：
Kojima, K;Hiratsuka, A;Karube, I
通讯作者：
Karube, I
影响因子：
7.4
作者：
Munge, B;Liu, GD;Wang, J
通讯作者：
Wang, J
影响因子：
7.4
作者：
HEINEMAN, WR;HALSALL, HB
通讯作者：
HALSALL, HB
影响因子：
--
作者：
Bensmail H;Haoudi A
通讯作者：
Haoudi A