Systems analysis of lactose metabolism in Trichoderma reesei identifies a lactose permease that is essential for cellulase induction.
Systems analysis of lactose metabolism in Trichoderma reesei identifies a lactose permease that is essential for cellulase induction.
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DOI：
10.1371/journal.pone.0062631
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kubicek CP
中科院分区：
文献类型：
--
作者：
Ivanova C;Bååth JA;Seiboth B;Kubicek CP
里氏木霉在自然界中定殖于腐朽的木材，并从植物生物质中代谢纤维素和半纤维素。工业上生产相应的酶以应用于生物燃料和生物精炼工业。然而，这些酶也在乳糖（1，4 -0-β-d-吡喃半乳糖基-d-葡萄糖）存在下被诱导，乳糖是来自奶酪制造或乳清加工工业的废物。事实上，乳糖是T. reesei在工业水平上，但这种独特现象的原因尚不清楚。为了回答这个问题，我们用系统分析的T。reesei转录组在乳糖利用过程中。我们发现，相应的CAZome编码纤维素降解所必需的所有糖基水解酶，特别是对于单子叶植物木葡聚糖的攻击，从中可以释放β-半乳糖苷，其可以作为T.里氏纤维素酶和半纤维素酶。此外，乳糖还诱导大量的主要易化超家族的推定转运蛋白。其中14个基因的缺失鉴定出一个基因，该基因对乳糖的利用和乳糖的吸收以及乳糖（而不是槐糖）对纤维素酶的诱导是必不可少的。reesei。这些数据揭示了T. reesei代谢乳糖并提供改善乳糖代谢的策略。它们还阐明了β-D-半乳糖苷在该真菌的生境特异性中的关键作用。
Trichoderma reesei colonizes predecayed wood in nature and metabolizes cellulose and hemicellulose from the plant biomass. The respective enzymes are industrially produced for application in the biofuel and biorefinery industry. However, these enzymes are also induced in the presence of lactose (1,4-0-ß-d-galactopyranosyl-d-glucose), a waste product from cheese manufacture or whey processing industries. In fact, lactose is the only soluble carbon source that induces these enzymes in T. reesei on an industrial level but the reason for this unique phenomenon is not understood. To answer this question, we used systems analysis of the T. reesei transcriptome during utilization of lactose. We found that the respective CAZome encoded all glycosyl hydrolases necessary for cellulose degradation and particularly for the attack of monocotyledon xyloglucan, from which ß-galactosides could be released that may act as the inducers of T. reesei’s cellulases and hemicellulases. In addition, lactose also induces a high number of putative transporters of the major facilitator superfamily. Deletion of fourteen of them identified one gene that is essential for lactose utilization and lactose uptake, and for cellulase induction by lactose (but not sophorose) in pregrown mycelia of T. reesei. These data shed new light on the mechanism by which T. reesei metabolizes lactose and offers strategies for its improvement. They also illuminate the key role of ß-D-galactosides in habitat specificity of this fungus.
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在真菌trichoderma resei和酿酒酵母中对分泌应激的转录反应的共同特征和有趣的差异。
影响因子：
4.4
作者：
Arvas M;Pakula T;Lanthaler K;Saloheimo M;Valkonen M;Suortti T;Robson G;Penttilä M
通讯作者：
Penttilä M
影响因子：
2.5
作者：
GRUBER, F;VISSER, J;DEGRAAFF, LH
通讯作者：
DEGRAAFF, LH
影响因子：
--
作者：
Metz, Benjamin;Seidl-Seiboth, Verena;Kubicek, Christian P.
通讯作者：
Kubicek, Christian P.
DOI：
10.1073/pnas.1200785109
发表时间：
2012-05-08
影响因子：
11.1
作者：
Coradetti, Samuel T.;Craig, James P.;Glass, N. Louise
通讯作者：
Glass, N. Louise
影响因子：
--
作者：
Akel, Eda;Metz, Benjamin;Kubicek, Christian P.
通讯作者：
Kubicek, Christian P.