Distinctive probiotic features share common TLR2-dependent signalling in intestinal epithelial cells.
Distinctive probiotic features share common TLR2-dependent signalling in intestinal epithelial cells.
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DOI：
10.1111/cmi.13264
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发表时间：
2021-01
影响因子：
3.4
通讯作者：
Rogelj I
中科院分区：
文献类型：
--
作者：
Paveljšek D;Ivičak-Kocjan K;Treven P;Benčina M;Jerala R;Rogelj I
益生菌和益生素的潜在机制尚不清楚，但已知两者都会影响适应性和先天性免疫反应。此外，越来越多的人认为，一些益生菌菌株具有共同的核心机制，可以提供某些健康益处。在此，我们的目的是阐明益生菌菌株副乳杆菌K7、发酵硅酸乳杆菌L930 BB、动物双歧杆菌亚种L930 BB和乳酸杆菌L930 BB的信号传导。animalis IM 386和植物乳杆菌WCFS 1。我们在体外实验中表明，受试益生菌表现出共同的TLR 2和TLR 10依赖性下游信号级联，涉及抑制NF-κB信号转导。在炎症条件下，益生菌激活磷脂酰肌醇3激酶（PI 3 K）/Akt抗凋亡途径和蛋白激酶C（PKC）依赖性途径，从而导致肌动蛋白细胞骨架和紧密连接的调节。这些途径有助于肠上皮的再生和粘膜免疫系统的调节，其与典型TLR信号传导的抑制一起促进一般免疫耐受。通过这项研究，我们确定了共享的益生菌机制，并首次通过TLR 10确定了抗炎益生菌信号传导的作用。
The underlying mechanisms of probiotics and postbiotics are not well understood, but it is known that both affect the adaptive and innate immune responses. In addition, there is a growing concept that some probiotic strains have common core mechanisms that provide certain health benefits. Here, we aimed to elucidate the signalization of the probiotic bacterial strains Lactobacillus paragasseri K7, Limosilactobacillus fermentum L930BB, Bifidobacterium animalis subsp. animalis IM386 and Lactiplantibacillus plantarum WCFS1. We showed in in vitro experiments that the tested probiotics exhibit common TLR2‐ and TLR10‐dependent downstream signalling cascades involving inhibition of NF‐κB signal transduction. Under inflammatory conditions, the probiotics activated phosphatidylinositol 3‐kinase (PI3K)/Akt anti‐apoptotic pathways and protein kinase C (PKC)‐dependent pathways, which led to regulation of the actin cytoskeleton and tight junctions. These pathways contribute to the regeneration of the intestinal epithelium and modulation of the mucosal immune system, which, together with the inhibition of canonical TLR signalling, promote general immune tolerance. With this study we identified shared probiotic mechanisms and were the first to pinpoint the role of anti‐inflammatory probiotic signalling through TLR10.
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DOI：
10.4049/jimmunol.1502599
发表时间：
2016-05-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Jiang S;Li X;Hess NJ;Guan Y;Tapping RI
通讯作者：
Tapping RI
影响因子：
1.2
作者：
Baeuerl, Christine;Perez-Martinez, Gaspar;Monedero, Vicente
通讯作者：
Monedero, Vicente
影响因子：
13.6
作者：
Jiménez-Dalmaroni MJ;Gerswhin ME;Adamopoulos IE
通讯作者：
Adamopoulos IE
影响因子：
32.4
作者：
Price AE;Shamardani K;Lugo KA;Deguine J;Roberts AW;Lee BL;Barton GM
通讯作者：
Barton GM
肠道菌群与溃疡性结肠炎的关系：益生菌和粪便菌群移植的机制及临床应用
影响因子：
4.3
作者：
Shen ZH;Zhu CX;Quan YS;Yang ZY;Wu S;Luo WW;Tan B;Wang XY
通讯作者：
Wang XY