Extreme RNA editing in coding islands and abundant microsatellites in repeat sequences of Selaginella moellendorffii mitochondria: the root of frequent plant mtDNA recombination in early tracheophytes.
Extreme RNA editing in coding islands and abundant microsatellites in repeat sequences of Selaginella moellendorffii mitochondria: the root of frequent plant mtDNA recombination in early tracheophytes.
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DOI:
10.1093/gbe/evr027
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发表时间:
2011
影响因子:
3.3
通讯作者:
Knoop V
中科院分区:
文献类型:
--
作者:
Hecht J;Grewe F;Knoop V
Using an independent fosmid cloning approach and comprehensive transcriptome analysis to complement data from the Selaginella moellendorffii genome project, we determined the complete mitochondrial genome structure of this spikemoss. Numerous recombination events mediated mainly via long sequence repeats extending up to 7kbp result in a complex mtDNA network structure. Peculiar features associated with the repeat sequences are more than 80 different microsatellite sites (predominantly trinucleotide motifs). The S. moellendorffii mtDNA encodes a plant-typical core set of a twin-arginine translocase (tatC), 17 respiratory chain subunits, and 2 rRNAs but lacks atp4 and any tRNA genes. As a further novelty among plant chondromes, the nad4L gene is encoded within an intron of the nad1 gene. A total of 37 introns occupying the 20 mitochondrial genes (four of which are disrupted into trans-splicing arrangements including two novel instances of trans-splicing introns) make the S. moellendorffii chondrome the intron-richest and gene-poorest plant mtDNA known. Our parallel transcriptome analyses demonstrates functional splicing of all 37 introns and reveals a new record amount of plant organelle RNA editing with a total of 2,139 sites in mRNAs and 13 sites in the two rRNAs, all of which are exclusively of the C-to-U type.
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影响因子:
10.7
作者:
Chaw, Shu-Miaw;Shih, Arthur Chun-Chieh;Chou, The-Yuan
通讯作者:
Chou, The-Yuan
影响因子:
10.7
作者:
Groth-Malonek, Milena;Wahrmund, Ute;Knoop, Volker
通讯作者:
Knoop, Volker
DOI:
10.1073/pnas.042694899
发表时间:
2002-07-23
影响因子:
11.1
作者:
Adams, KL;Qiu, YL;Palmer, JD
通讯作者:
Palmer, JD
影响因子:
10.7
作者:
Alverson, Andrew J.;Wei, XiaoXin;Palmer, Jeffrey D.
通讯作者:
Palmer, Jeffrey D.
影响因子:
14.9
作者:
Grewe F;Viehoever P;Weisshaar B;Knoop V
通讯作者:
Knoop V