Lateral Flow Glyco-Assays for the Rapid and Low-Cost Detection of Lectins-Polymeric Linkers and Particle Engineering Are Essential for Selectivity and Performance.
Lateral Flow Glyco-Assays for the Rapid and Low-Cost Detection of Lectins-Polymeric Linkers and Particle Engineering Are Essential for Selectivity and Performance.
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DOI：
10.1002/adhm.202101784
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发表时间：
2022-03
影响因子：
10
通讯作者：
Gibson MI
中科院分区：
文献类型：
--
作者：
Baker AN;Muguruza AR;Richards SJ;Georgiou PG;Goetz S;Walker M;Dedola S;Field RA;Gibson MI
侧流免疫测定，如家庭妊娠测试，是快速的护理点诊断，其使用抗体包被的纳米颗粒结合抗原/分析物（例如，病毒、毒素或激素）。易于使用，无需集中式基础设施和低成本，使这些设备对快速疾病识别具有吸引力，特别是在低资源环境中。在这里，糖基化的聚合物包覆的纳米颗粒被证明用于在侧向流动和流过中的凝集素的灵敏的、无标记的检测。这里介绍的系统使用聚糖，而不是抗体，以提供识别：“侧流糖测定”，提供独特的生物传感机会。聚糖被安装在聚合物末端并固定在金纳米颗粒上，提供胶体稳定性，但关键的是还引入了测定可调性和选择性。使用大豆凝集素和蓖麻凝集素I（RCA 120）作为模型分析物，聚合物链长和纳米颗粒核心大小的影响进行了评估，发现链长对信号产生有显着影响，突出了需要控制大分子结构来调节响应。使用优化的系统，凝集素可在亚纳摩尔浓度下检测到，与基于抗体的系统相当。完整的侧流装置也组装，以显示这些设备可以部署在“真实的世界”。这项工作表明，聚糖结合可以是一个有价值的工具，在快速诊断。
Lateral flow immuno-assays, such as the home pregnancy test, are rapid point-of-care diagnostics that use antibody-coated nanoparticles to bind antigens/analytes (e.g., viruses, toxins or hormones). Ease of use, no need for centralized infrastructure and low-cost, makes these devices appealing for rapid disease identification, especially in low-resource environments. Here glycosylated polymer-coated nanoparticles are demonstrated for the sensitive, label-free detection of lectins in lateral flow and flow-through. The systems introduced here use glycans, not antibodies, to provide recognition: a “lateral flow glyco-assay,” providing unique biosensing opportunities. Glycans are installed onto polymer termini and immobilized onto gold nanoparticles, providing colloidal stability but crucially also introducing assay tunability and selectivity. Using soybean agglutinin and Ricinus communis agglutinin I (RCA120) as model analytes, the impact of polymer chain length and nanoparticle core size are evaluated, with chain length found to have a significant effect on signal generation—highlighting the need to control the macromolecular architecture to tune response. With optimized systems, lectins are detectable at subnanomolar concentrations, comparable to antibody-based systems. Complete lateral flow devices are also assembled to show how these devices can be deployed in the “real world.” This work shows that glycan-binding can be a valuable tool in rapid diagnostics.
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影响因子：
8.9
作者：
Baker AN;Richards SJ;Pandey S;Guy CS;Ahmad A;Hasan M;Biggs CI;Georgiou PG;Zwetsloot AJ;Straube A;Dedola S;Field RA;Anderson NR;Walker M;Grammatopoulos D;Gibson MI
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发表时间：
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影响因子：
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影响因子：
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