Richardson-Lucy deconvolution as a general tool for combining images with complementary strengths.

Richardson-Lucy deconvolution as a general tool for combining images with complementary strengths.
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Richardson-Lucy Deoncolution是将图像与互补强度结合的一般工具。

DOI:
10.1002/cphc.201300831
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发表时间:
2014-03-17
期刊:
Chemphyschem : a European journal of chemical physics and physical chemistry
影响因子:
--
通讯作者:
Patterson GH
Patterson GH
中科院分区:
其他
文献类型:
--
作者:
Ingaramo M;York AG;Hoogendoorn E;Postma M;Shroff H;Patterson GH

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We use Richardson-Lucy (RL) deconvolution to combine multiple images of a simulated object into a single image in the context of modern fluorescence microscopy techniques. RL deconvolution can merge images with very different point spread functions, such as in multiview light-sheet microscopes , while preserving the best resolution information present in each image. We show RL deconvolution is also easily applied to merge high-resolution, high noise images with low-resolution, low noise images, relevant when complementing conventional microscopy with localization microscopy. We also use RL deconvolution to merge images produced via different simulated illumination patterns, relevant to structured illumination microscopy (SIM) and image scanning microscopy (ISM). The quality of our ISM reconstructions are at least as good as standard inversion algorithms for ISM data, but our method follows a simpler recipe that requires no mathematical insight. Finally, we apply RL deconvolution to merge a series of ten images with varying signal and resolution levels. This combination is relevant to gated stimulated-emission depletion (STED) microscopy, and shows that high-quality image merges are possible even in cases where a non-iterative inversion algorithm is unknown.
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