Aging Affects the Efficacy of Platelet-Rich Plasma Treatment for Osteoarthritis.
Aging Affects the Efficacy of Platelet-Rich Plasma Treatment for Osteoarthritis.
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DOI：
10.1097/phm.0000000000002161
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发表时间：
2023-07-01
影响因子：
3
通讯作者：
中科院分区：
文献类型：
--
作者：
Despite the increased use of platelet-rich plasma in the treatment of osteoarthritis, whether and how age of the platelet-rich plasma donor affects therapeutic efficacy is unclear. In vitro, male osteoarthritic human chondrocytes were treated with platelet-rich plasma from young (18–35 yrs) or old (≥65 yrs) donors, and the chondrogenic profile was evaluated using immunofluorescent staining for two markers of chondrogenicity, type II collagen and SOX-9. In vivo, we used a within-subjects design to compare Osteoarthritis Research Society International scores in aged mouse knee joints injected with platelet-rich plasma from young or old individuals. In vitro experiments revealed that platelet-rich plasma from young donors induced a more youthful chondrocyte phenotype, as evidenced by increased type II collagen (P = 0.033) and SOX-9 expression (P = 0.022). This benefit, however, was significantly blunted when cells were cultured with platelet-rich plasma from aged donors. Accordingly, in vivo studies revealed that animals treated with platelet-rich plasma from young donors displayed a significantly improved cartilage integrity when compared with knees injected with platelet-rich plasma from aged donors (P = 0.019). Injection of platelet-rich plasma from a young individual induced a regenerative effect in aged cells and mice, whereas platelet-rich plasma from aged individuals showed no improvement in chondrocyte health or cartilage integrity.
影响因子：
2.9
作者：
Li L;Wei X;Geng X;Duan Z;Wang X;Li P;Wang C;Wei L
通讯作者：
Wei L
影响因子：
3.9
作者：
Trevissón B;Becerro-de-Bengoa-Vallejo R;Sevillano D;González N;Losa-Iglesias ME;López-López D;Alou L
通讯作者：
Alou L