The guanine-quadruplex structure in the human c-myc gene's promoter is converted into B-DNA form by the human poly(ADP-ribose)polymerase-1.
The guanine-quadruplex structure in the human c-myc gene's promoter is converted into B-DNA form by the human poly(ADP-ribose)polymerase-1.
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DOI：
10.1371/journal.pone.0042690
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Bauer PI
中科院分区：
文献类型：
--
作者：
Fekete A;Kenesi E;Hunyadi-Gulyas E;Durgo H;Berko B;Dunai ZA;Bauer PI
存在于人类c-myc （h c-myc）基因启动子核酸酶超敏元件（NHE） III1区域的鸟嘌呤四重体（GQ）结构在该基因转录调控中的重要调控作用已被证实。本研究通过结合实验、荧光能量转移（FRET）实验和亲和下拉实验，以及染色质免疫沉淀(ChIP)-qPCR分析和体内h - c-myc启动子荧光素酶报告基因检测，证明人核聚（adp -核糖）聚合酶1 （h PARP-1）蛋白通过与GQ结构的相互作用参与了h - c-myc基因表达的调控。我们推测PARP-1与GQ结构结合，并参与将该结构转化为转录活性更高的B-DNA形式。PARP-1中存在的第一个锌指结构参与了这种相互作用。PARP-1可能是通过与不同基因启动子中的GQ结构相互作用参与转录调控的蛋白组中的新成员。
The important regulatory role of the guanine-quadruplex (GQ) structure, present in the nuclease hypersensitive element (NHE) III1 region of the human c-myc (h c-myc) gene's promoter, in the regulation of the transcription of that gene has been documented. Here we present evidences, that the human nuclear poly(ADP-ribose)polymerase-1 (h PARP-1) protein participates in the regulation of the h c-myc gene expression through its interaction with this GQ structure, characterized by binding assays, fluorescence energy transfer (FRET) experiments and by affinity pull-down experiments in vitro, and by chromatin immunoprecipitation (ChIP)-qPCR analysis and h c-myc-promoter-luciferase reporter determinations in vivo. We surmise that h PARP-1 binds to the GQ structure and participates in the conversion of that structure into the transcriptionally more active B-DNA form. The first Zn-finger structure present in h PARP-1 participates in this interaction. PARP-1 might be a new member of the group of proteins participating in the regulation of transcription through their interactions with GQ structures present in the promoters of different genes.
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影响因子：
4.8
作者：
BUKI, KG;BAUER, PI;KUN, E
通讯作者：
KUN, E
影响因子：
3.2
作者：
IKAI, K;UEDA, K
通讯作者：
UEDA, K
影响因子：
4.8
作者：
Balakrishnan, Lata;Polaczek, Piotr;Bambara, Robert A.
通讯作者：
Bambara, Robert A.
影响因子：
56.9
作者：
He, TC;Sparks, AB;Kinzler, KW
通讯作者：
Kinzler, KW
DOI：
10.1111/j.1742-4658.2010.07759.x
发表时间：
2010-09
期刊：
The FEBS journal
影响因子：
--
作者：
Brooks TA;Kendrick S;Hurley L
通讯作者：
Hurley L