Discrepancies between patterns of potentially lethal damage repair in the RIF-1 tumor system in vitro and in vivo.
Discrepancies between patterns of potentially lethal damage repair in the RIF-1 tumor system in vitro and in vivo.
复制标题
RIF-1肿瘤系统体外和体内潜在致命损伤修复模式之间的差异。
DOI：
10.2307/3575951
复制
发表时间：
1983
影响因子：
3.4
通讯作者：
N. Nelson
中科院分区：
文献类型：
--
作者：
J. Rasey;N. Nelson
在RIF-1肿瘤系统中，研究了体内和体外几种不同生长状态下潜在致死损伤(PLD)的修复。研究了细胞培养中指数生长、饲养高原和未饲养的高原细胞以及皮下或肌肉内小的和大的肿瘤。研究了大剂量单次照射后不同的修复时间以及分级剂量的照射以在照射后立即或在完全修复后产生生存曲线。在体外研究的所有促进修复的条件(延迟传代培养，细胞在照射后暴露在耗尽的生长介质中)在单次剂量后都会增加存活率。细胞存活曲线的D0也因此而增加。在体外实验之前，没有观察到任何在体内照射并在动物体内保持不同时间的肿瘤的PLD修复。当该肿瘤被制备成单细胞悬浮液用于克隆形成时，获得了近100%的细胞产率，获得了具有代表性的细胞样本，并且每克细胞产量随照射后时间的变化而恒定，这表明这种差异不是检测系统的伪影。对这些关于肿瘤放射治疗能力的数据和信息最合理的解释是，在体外经常证明的PLD修复，当留在原位时，可能不是该肿瘤放射反应的主要因素。
Repair of potentially lethal damage (PLD) was studied in the RIF-1 tumor system in several different growth states in vivo and in vitro. Exponentially growing, fed plateau, and unfed plateau cells in cell culture as well as small and large subcutaneous or intramuscular tumors were investigated. Large single doses of radiation followed by variable repair times as well as graded doses of radiation to generate survival curves immediately after irradiation or after full repair were investigated. All repair-promoting conditions studied in vitro (delayed subculture, exposure of cells to depleted growth medium after irradiation) increased surviving fraction after a single dose. The D0 of the cell survival curve was also increased by these procedures. No PLD repair was observed for any tumors irradiated in vivo and maintained in the animal for varying times prior to assay in vitro. The nearly 100% cell yield obtained when this tumor is prepared as a single-cell suspension for colony formation, the representative cell sample obtained, and the constant cell yield per gram as a function of time postirradiation suggest that this discrepancy is not an artifact of the assay system. The most logical explanation of these data and information on radiocurability of this neoplasm is that PLD repair, which is so frequently demonstrated in vitro, may not be a major factor in the radioresponse of this tumor when left in situ.
DNA 修复和染色体畸变与 X 射线照射哺乳动物细胞中潜在致命损伤修复的关系。
DOI：
10.1007/978-1-4684-3842-0_18
发表时间：
1980
期刊：
Basic life sciences
影响因子：
--
作者：
FornaceJr,AJ;Nagasawa,H;Little,JB
通讯作者：
Little,JB