Relationship of DNA repair and chromosome aberrations to potentially lethal damage repair in X-irradiated mammalian cells.
Relationship of DNA repair and chromosome aberrations to potentially lethal damage repair in X-irradiated mammalian cells.
复制标题
DNA 修复和染色体畸变与 X 射线照射哺乳动物细胞中潜在致命损伤修复的关系。
DOI：
10.1007/978-1-4684-3842-0_18
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Little,JB
中科院分区：
文献类型：
--
作者：
FornaceJr,AJ;Nagasawa,H;Little,JB
通过碱性洗脱技术，在稳定期、接触抑制的小鼠细胞中研究了 X 射线诱导的 DNA 单链断裂和 DNA-蛋白质交联的修复。在修复的第一个小时内，大约 90% 的 X 射线诱导的单链断裂重新连接，而大多数剩余断裂在接下来的 5 小时内重新连接得更慢。残留的未重新连接的单链断裂的数量大约与早期修复时的 X 射线剂量成正比。 DNA-蛋白质交联以较慢的速度去除 - T½ 大约 10-12 小时。细胞在辐射后的不同时间以低密度进行传代培养，并对集落存活率（潜在的致命损伤修复）和传代培养后第一次有丝分裂中的染色体畸变进行评分。在修复的最初几个小时内，细胞致死率和染色体畸变频率均下降，并在 6 小时时达到最低水平；这种减少在时间上与缓慢重新连接的 DNA 链断裂的修复相关。讨论了 DNA 修复与生存变化和染色体畸变的可能关系。
By the alkaline elution technique, the repair of x-ray-induced DNA single strand breaks and DNA-protein cross-links was investigated in stationary phase, contact-inhibited mouse cells. During the first hour of repair, approximately 90% of x-ray induced single strand breaks were rejoined whereas most of the remaining breaks were rejoined more slowly during the next 5 hr. The number of residual non-rejoined single strand breaks was approximately proportional to the x-ray dose at early repair times. DNA-protein cross-links were removed at a slower rate — T½ approximately 10–12 hr. Cells were subcultured at low density at various times after irradiation and scored for colony survival (potentially lethal damage repair), and chromosome aberrations in the first mitosis after sub-culture. Both cell lethality and the frequency of chromosome aberrations decreased during the first several hours of repair, reaching a minimum level by 6 hr; this decrease correlated temporally with the repair of the slowly rejoining DNA strand breaks. The possible relationship of DNA repair to changes in survival and chromosome aberrations is discussed.
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哺乳动物细胞中潜在致命辐射损伤的修复与恶性转化的增强有关
影响因子：
64.8
作者：
Margaret Terzaghi;J. Little
通讯作者：
J. Little
DOI：
10.1093/jnci/21.3.437
发表时间：
1958
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
T. Hsu;Oleta Klatt
通讯作者：
Oleta Klatt
影响因子：
5.6
作者：
FUJIWARA, Y;TATSUMI, M;SASAKI, MS
通讯作者：
SASAKI, MS
信件：早期修复过程中受辐射胸腺细胞 DNA 链断裂端基的修饰。
DOI：
10.1080/09553007514550921
发表时间：
1975
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
--
作者：
M. Lennartz;T. Coquerelle;U. Hagen
通讯作者：
U. Hagen
X 射线产生的噬菌体 DNA 发生致命变化。
影响因子：
3.4
作者：
D. Freifelder
通讯作者：
D. Freifelder