Structural library and visualization of endogenously oxidized phosphatidylcholines using mass spectrometry-based techniques.
Structural library and visualization of endogenously oxidized phosphatidylcholines using mass spectrometry-based techniques.
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DOI：
10.1038/s41467-021-26633-w
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发表时间：
2021-11-03
影响因子：
16.6
通讯作者：
Yamada KI
中科院分区：
文献类型：
--
作者：
Matsuoka Y;Takahashi M;Sugiura Y;Izumi Y;Nishiyama K;Nishida M;Suematsu M;Bamba T;Yamada KI
虽然氧化磷脂酰胆碱（oxPCs）在许多病理事件中起着关键作用，但由于缺乏结构信息和专门的分析方法，内源性oxPCs的类型和产生位点仍然未知。本文采用基于高分辨率质谱的非靶向分析方法构建了465个oxPCs文库，并用于检测对乙酰氨基酚诱导的急性肝衰竭小鼠中的70个oxPCs。我们发现双氧多不饱和脂肪酸(PUFA)-PC (PC PUFA;O2)，含有环氧和氢氧化物基团，在肝损伤早期产生。体内18O标记杂交和基质辅助激光解吸/电离串联质谱成像显示PC PUFA；O2在表达细胞色素P450 - 2e1和谷胱甘肽缺失的肝细胞中积累，这是肝损伤的主要部位。开发的文库和可视化方法将有助于表征特定的脂质过氧化事件，并增强我们对其在脂质过氧化相关疾病中的生理和病理意义的理解。氧化磷脂酰胆碱（oxPCs）是一类结构多样的脂类，与多种疾病相关。在这里，作者使用质谱法构建了465个oxpc的谱库，并随后分析了对乙酰氨基酚诱导的小鼠急性肝衰竭期间形成的oxpc。
Although oxidized phosphatidylcholines (oxPCs) play critical roles in numerous pathological events, the type and production sites of endogenous oxPCs remain unknown because of the lack of structural information and dedicated analytical methods. Herein, a library of 465 oxPCs is constructed using high-resolution mass spectrometry-based non-targeted analytical methods and employed to detect 70 oxPCs in mice with acetaminophen-induced acute liver failure. We show that doubly oxygenated polyunsaturated fatty acid (PUFA)-PCs (PC PUFA;O2), containing epoxy and hydroxide groups, are generated in the early phase of liver injury. Hybridization with in-vivo 18O labeling and matrix-assisted laser desorption/ionization-tandem MS imaging reveals that PC PUFA;O2 are accumulated in cytochrome P450 2E1-expressing and glutathione-depleted hepatocytes, which are the major sites of liver injury. The developed library and visualization methodology should facilitate the characterization of specific lipid peroxidation events and enhance our understanding of their physiological and pathological significance in lipid peroxidation-related diseases. Oxidized phosphatidylcholines (oxPCs) are a structurally diverse class of lipids associated with various diseases. Here, the authors use mass spectrometry to construct a spectral library of 465 oxPCs and subsequently profile oxPCs formed during acetaminophen-induced acute liver failure in mice.
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影响因子：
3.9
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1007/s13361-011-0074-3
发表时间：
2011-04-01
影响因子：
3.2
作者：
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通讯作者：
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影响因子：
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作者：
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