Efficiency of RFLP, RAPD, and AFLP markers for the construction of an intraspecific map of the tomato genome.
Efficiency of RFLP, RAPD, and AFLP markers for the construction of an intraspecific map of the tomato genome.
复制标题
RFLP、RAPD 和 AFLP 标记用于构建番茄基因组种内图谱的效率。
作者：
V. Saliba;M. Causse;L. Gervais;J. Philouze
我们基于番茄和番茄变种的两个自交系之间的种内杂交构建了番茄遗传连锁图谱。樱桃形。分离群体由 153 个重组自交系组成。该图谱由 1 个形态学位点、132 个 RFLP（限制性片段长度多态性，包括 16 个已知功能基因）、33 个 RAPD（随机扩增多态性 DNA）和 211 个 AFLP（扩增片段长度多态性）基因座组成。我们比较了 3 种标记的多态性、分离和在基因组中的分布。 RFLP、RAPD 和 AFLP 方法分别显示 8.7%、15.8% 和 14.5% 的信息带。这对应于 30% 的 RFLP 探针、32% 的 RAPD 引物和 100% 的 AFLP 引物组合的多态性。 RFLP 与 AFLP 位点相比，与 1:1 预期比率的偏差较小（分别为 8% 和 18%）。 RAPD 和 AFLP 标记并非随机分布在基因组中。其中大多数（分别为 60% 和 80%）聚集在假定的着丝粒区域周围。该种内图谱跨度为 965 cM，（框架图谱）标记之间的平均距离为 8.3 cM。将其与其他已发表的番茄种间图谱进行了比较。尽管该图谱具有种内起源，但与番茄的高密度种间图谱相比，它的长度并未显示出任何增加。
We have constructed a tomato genetic linkage map based on an intraspecific cross between two inbred lines of Lycopersicon esculentum and L. esculentum var. cerasiforme. The segregating population was composed of 153 recombinant inbred lines. This map is comprised of one morphological, 132 RFLP (restriction fragment length polymorphism, including 16 known-function genes), 33 RAPD (random amplified polymorphic DNA), and 211 AFLP (amplified fragment length polymorphism) loci. We compared the 3 types of markers for their polymorphism, segregation, and distribution over the genome. RFLP, RAPD, and AFLP methods revealed 8.7%, 15.8%, and 14.5% informative bands, respectively. This corresponded to polymorphism in 30% of RFLP probes, 32% of RAPD primers, and 100% of AFLP primer combinations. Less deviation from the 1:1 expected ratio was obtained with RFLP than with AFLP loci (8% and 18%, respectively). RAPD and AFLP markers were not randomly distributed over the genome. Most of them (60% and 80%, respectively) were grouped in clusters located around putative centromeric regions. This intraspecific map spans 965 cM with an average distance of 8.3 cM between markers (of the framework map). It was compared to other published interspecific maps of tomato. Despite the intraspecific origin of this map, it did not show any increase in length when compared to the high-density interspecific map of tomato.
DOI：
10.1111/j.1365-313x.1992.00153.x
发表时间：
1992-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Lifang Shi;Robert T. Gast;Manjula Gopalraj;Neil E. Olszewski
通讯作者：
Lifang Shi;Robert T. Gast;Manjula Gopalraj;Neil E. Olszewski