Specializations of gastrin-releasing peptide cells of the mouse suprachiasmatic nucleus.
Specializations of gastrin-releasing peptide cells of the mouse suprachiasmatic nucleus.
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DOI：
10.1002/cne.22272
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发表时间：
2010-04-15
期刊：
影响因子：
--
通讯作者：
Silver R
中科院分区：
文献类型：
--
作者：
Drouyer E;LeSauter J;Hernandez AL;Silver R
下丘脑的视交叉上核(SCN)调节生理和行为的日常节律。它由一组不同种类的单元组成，这些单元共同构成其主时钟功能的基础电路。大量研究表明，存在两个区域，分别称为核区和壳区。在总体水平上，这些区域之间的差异映射到明显的功能差异，尽管各种肽能细胞表型的具体作用(S)仍不清楚。在小鼠中，胃泌素释放肽(GRP)细胞处于核心位置，直接依赖视网膜，在时钟基因表达中缺乏可检测到的节律性，从而引起了人们对它们在SCN中作用的兴趣。在这里，我们提供了围产期小鼠SCN表达Calbindin的细胞表达GRP的证据，该GRP由绿色荧光蛋白(GFP+)鉴定，但在发育后期缺乏可检测到的Calbindin。为了探索GRP神经元参与的SCN内网络，用示踪剂填充单个GFP+细胞，比较它们的形态特征、突起和连接，以及它们与含有GFP的非GFP细胞的联系。结果表明，GFP+神经元在核内形成致密的局部电路网络，表现为与其他GFP+细胞的结合和染料偶联细胞的存在。GFP+细胞的树突和轴突与精氨酸加压素神经元结合，而非GFP+细胞的纤维网络较少，主要局限于GFP+细胞区域。研究结果表明，SCN内有专门的电路，可能支持神经活动的同步以及核心区和壳区之间的相互通信。
The suprachiasmatic nucleus (SCN) of the hypothalamus regulates daily rhythms in physiology and behavior. It is constituted of a heterogeneous population of cells which together form the circuits underlying its master clock function. Numerous studies suggest the existence of two regions that have been termed core and shell. At a gross level, differences between these regions map to distinct functional differences, though the specific role(s) of various peptidergic cellular phenotypes remains unknown. In mouse, gastrin releasing peptide (GRP) cells lie in the core, are directly retinorecipient and lack detectable rhythmicity in clock gene expression, raising interest in their role in the SCN. Here, we provide evidence that calbindin expressing cells of perinatal mouse SCN express GRP, identified by a green fluorescent protein (GFP+), but lack detectable calbindin later in development. To explore the intra-SCN network in which GRP neurons participate, individual GFP+ cells were filled with tracer and their morphological characteristics, processes, and connections, as well as those of their non-GFP containing immediate neighbors, were compared. The results show that GFP+ neurons form a dense network of local circuits within the core, revealed by appositions on other GFP+ cells and by the presence of dye-coupled cells. Dendrites and axons of GFP+ cells make appositions on arginine vasopressin neurons, while non-GFP cells have a less extensive fiber network, largely confined to the region of GFP+ cells. The results point to specialized circuitry within the SCN, presumably supporting synchronization of neural activity and reciprocal communication between core and shell regions.
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影响因子：
3.4
作者：
Karatsoreos, IN;Romeo, RD;Silver, R
通讯作者：
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3.3
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