Lipidic Cubic Phase-Induced Membrane Protein Crystallization: Interplay Between Lipid Molecular Structure, Mesophase Structure and Properties, and Crystallogenesis
Lipidic Cubic Phase-Induced Membrane Protein Crystallization: Interplay Between Lipid Molecular Structure, Mesophase Structure and Properties, and Crystallogenesis
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脂质立方相诱导膜蛋白结晶：脂质分子结构、中间相结构和性质以及结晶发生之间的相互作用
DOI：
10.1021/acs.cgd.7b00519
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发表时间：
2017
影响因子：
3.8
通讯作者：
C. Drummond
中科院分区：
文献类型：
--
作者：
Alexandru Zabara;T. Meikle;R. Trenker;S. Yao;J. Newman;T. Peat;F. Separovic;C. Conn;M. J. Call;M. Call;E. Landau;C. Drummond
获得衍射性良好的膜蛋白晶体，对于在分子水平上理解其内在的三维结构、动力学和功能至关重要，是结构生物学领域的一个基本瓶颈。膜蛋白结构测定领域的主要进展之一是认识到纳米结构脂质立方相(LCP)环境构成了膜模拟基质，促进了特定膜蛋白的增溶、稳定和结晶。尽管基于LCP的膜蛋白结晶的引入已经过去了20年，但研究界对驱动LCP中蛋白质成核和大分子组装的过程的了解总体上仍然有限。在目前的研究中，我们提出了一个详细的，系统的研究之间的关系，脂类的化学结构，随后的中间相的物理性质，平移扩散…
Obtaining well-diffracting crystals of membrane proteins, which is crucial to the molecular-level understanding of their intrinsic three-dimensional structure, dynamics, and function, represents a fundamental bottleneck in the field of structural biology. One of the major advances in the field of membrane protein structural determination was the realization that the nanostructured lipidic cubic phase (LCP) environment constitutes a membrane mimetic matrix that promotes solubilization, stabilization, and crystallization of specific membrane proteins. Despite two decades passing since the introduction of LCP-based membrane protein crystallization, the research community’s understanding of the processes that drive protein nucleation and macromolecular assembly in the LCP generally remains limited. In the current study, we present a detailed, systematic investigation into the relationship between the chemical structure of the lipid, the physical properties of the ensuing mesophase, the translational diffusion...
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影响因子：
3.8
作者：
Li, Dianfan;Lee, Jean;Caffrey, Martin
通讯作者：
Caffrey, Martin
影响因子：
5.6
作者：
Cherezov, V.;Yamashita, E.;Caffrey, M.
通讯作者：
Caffrey, M.
影响因子：
3.8
作者：
Cherezov, Vadim;Liu, Jeffrey;Stevens, Raymond C.
通讯作者：
Stevens, Raymond C.
影响因子：
5.7
作者：
Fairman, James W.;Dautin, Nathalie;Wojtowicz, Damian;Liu, Wei;Noinaj, Nicholas;Barnard, Travis J.;Udho, Eshwar;Przytycka, Teresa M.;Cherezov, Vadim;Buchanan, Susan K.
通讯作者：
Buchanan, Susan K.
影响因子：
3.9
作者：
Caffrey M
通讯作者：
Caffrey M