Molecular plasticity of adult Bergmann fibers is associated with radial migration of grafted Purkinje cells
Molecular plasticity of adult Bergmann fibers is associated with radial migration of grafted Purkinje cells
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成年伯格曼纤维的分子可塑性与移植浦肯野细胞的径向迁移有关
DOI：
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发表时间：
1994
影响因子：
5.3
通讯作者：
Muriel Verner
中科院分区：
文献类型：
--
作者：
Constantino Sotelo;R. Alvarado;Monique Frain;Muriel Verner
来自小脑原基的胚胎浦肯野细胞（PCs）移植到成年pcd突变小脑中，取代宿主缺失的PCs，并成为突触整合到有缺陷的小脑回路中。这个神经元替代的过程开始于移植物的pc侵入宿主小脑，并通过其分子层径向迁移。本研究的目的是确定这种迁移的胶质轴是与移植的PCs一起迁移的胚胎放射状胶质细胞，还是宿主的成年伯格曼纤维，短暂地重新表达指导迁移所需的分子线索。通过lacZ表达鉴定伯格曼纤维的转基因小鼠（Krox- 20/lacZ14）的移植物表明，尽管在移植物残体和宿主分子层的移植物中存在x- gal染色的伯格曼纤维，但宿主小脑中的所有伯格曼纤维都缺乏β -半乳糖苷酶活性。因此，这些迁移轴属于寄主，而不属于供体。来自正常等基因小鼠胚胎的移植表明，在移植PCs的径向迁移过程中（移植后7 d），参与的宿主伯格曼纤维重新表达巢蛋白（通过单克隆抗体Rat-401免疫染色鉴定），通常仅由未成熟的伯格曼纤维表达。5天后，当移植的pc停止迁移时，宿主伯格曼纤维再次变为Rat-401阴性。这些结果表明，胚胎pc可以在成年小脑中触发其自身迁移和最终突触整合所必需的分子变化。
Embryonic Purkinje cells (PCs) from cerebellar primordia grafted in adult pcd mutant cerebellum replace missing PCs of the host, and become synaptically integrated into the defective cerebellar circuit. This process of neuronal replacement starts with the invasion of grafted PCs into the host cerebellum, and their radial migration through its molecular layer. The present study is aimed at determining whether the glial axes for this migration are embryonic radial glial cells that comigrate with the grafted PCs, or adult Bergmann fibers of the host, transiently reexpressing the molecular cues needed for their guidance of the migration. Transplants from a transgenic mouse line (Krox- 20/lacZ14) in which Bergmann fibers could be identified by lacZ expression reveal that, despite the presence of X-gal-stained Bergmann fibers in the graft remnants and of grafted PCs in the host molecular layer, all Bergmann fibers in the host cerebellum lack of beta- galactosidase activity. Thus, these migratory axes belong to the host, not to the donor. Transplants from normal isogenic mouse embryos show that during the radial migration of grafted PCs (7 d after grafting) the involved host Bergmann fibers reexpress nestin (identified with monoclonal antibody Rat-401 immunostaining), normally expressed only by immature Bergmann fibers. Five days later, when grafted PCs have arrested their migration, host Bergmann fibers again become Rat-401 negative. These results indicate that embryonic PCs can trigger in adult cerebellum the molecular changes necessary for their own migration and ultimate synaptic integration in the host cortical circuitry.
DOI：
10.1016/0165-3806(88)90121-6
发表时间：
1988-11-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
MISSON, JP;EDWARDS, MA;CAVINESS, VS
通讯作者：
CAVINESS, VS