Prokaryotic expression of partial E gene of DENV strain and its effect on HUVEC permeability
Prokaryotic expression of partial E gene of DENV strain and its effect on HUVEC permeability
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DENV毒株部分E基因的原核表达及其对HUVEC通透性的影响
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Li Zuo
中科院分区：
文献类型：
--
作者：
Jing Yuan;Bingle Lv;Li Zuo
登革热病毒（DENV）已成为世界范围内主要的公共卫生问题之一。热带和亚热带地区。E蛋白是大肠杆菌最大的结构蛋白和主要的包膜蛋白。病毒粒子，它可以诱导保护性免疫反应和随后的免疫病理损伤。病理学。DENV感染的特点是血管通透性增加。然而，蛋白E对。血管渗透性还没有被很好地理解。我们于2002年和2007年从白纹伊蚊中分离出DENV2 （M株）。测序结果为1 ~ 476 bp的E基因(GenBank No.；AY278226)。本实验制备了E基因1-476融合蛋白。DENV-2 M株和NGC株的序列，分别命名为M476和N476，及其各自的多克隆抗体。人脐静脉内皮细胞（HUVEC）与重组蛋白共培养体系，间接。免疫荧光染色显示M和NGC重组蛋白能与HUVEC结合。通过检测FITC荧光变化，发现M476蛋白对细胞膜通透性有显著影响。HUVEC在0.5~24 h和32~48 h， 12 h达到峰值。N476蛋白在0.5~48 h起作用，3 h起作用。与未处理的细胞相比，M476或N476处理后的HUVEC细胞形态学改变。蛋白质表现为细胞萎缩，细胞间宽度变宽，细胞连接中断。综合来看，我们的工作。有力地证实了DENV-2蛋白E对血管通透性的影响，这可能有助于了解血管通透性的变化。DENV的发病机制
Dengue virus (DENV) has become the one of the major public health problems worldwide, especially in.Tropical and subtropical regions. E protein is the biggest structural proteins and the major envelope protein of the.virion, which can induce protective immune response and subsequently immune pathological damage. The pathology.of DENV infection is characterized as increased vascular permeability. However, the effect of Protein E on the.vascular permeability is not well understood. We isolated DENV2 (M strain) from Aedes albopictus in 2002 and.sequenced 1-476 bp of its E gene (GenBank No. AY278226). Here we prepared fusion protein of E gene 1-476.sequence of DENV-2 M strain and NGC strain, named M476 and N476 respectively, and their respective polyclonal.antibody. In co-culture system of human umbilical vein endothelial cell (HUVEC) and recombinant protein, indirect.immunofluorescence staining showed that the recombinant protein of M and NGC can bind to HUVEC. Through.detecting FITC fluorescence change, it was found that M476 protein had significant effect on the permeability of.HUVEC during the period of 0.5~24 h and 32~48 h, 12 h at the peak. As for N476 protein, it functioned at 0.5~48.h, 3 h at the peak. Compared with untreated cells, the morphology of HUVEC cells treated with M476 or N476.protein showed shrinking cell, wider width between cells and disrupted cell connection. Taken together, our work.strongly confirmed the effect of protein E of DENV-2 on vascular permeability, which might help understanding the.pathogenesis of DENV in depth.
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影响因子：
5.4
作者：
Bhardwaj, S;Holbrook, M;Watowich, SJ
通讯作者：
Watowich, SJ
影响因子：
5.4
作者：
Stiasny, Karin;Kiermayr, Stefan;Heinz, Franz X.
通讯作者：
Heinz, Franz X.
DOI：
--
发表时间：
2015
期刊：
--
影响因子：
--
作者：
Jr;S. Barratt-Boyes;Sean P Mcburney;Jared D. Evans;E. Marques;Amanda P Nascimento;Klécia M Smith;Tatiana M Soares De Melo;Marli T Garcia-Bates;Eduardo J M Cordeiro
通讯作者：
Jr;S. Barratt-Boyes;Sean P Mcburney;Jared D. Evans;E. Marques;Amanda P Nascimento;Klécia M Smith;Tatiana M Soares De Melo;Marli T Garcia-Bates;Eduardo J M Cordeiro
影响因子：
5.4
作者：
W. Wahala;C. Huang;S. Butrapet;L. White;A. D. de Silva
通讯作者：
W. Wahala;C. Huang;S. Butrapet;L. White;A. D. de Silva
影响因子：
3.8
作者：
Hang VT;Nguyet NM;Trung DT;Tricou V;Yoksan S;Dung NM;Van Ngoc T;Hien TT;Farrar J;Wills B;Simmons CP
通讯作者：
Simmons CP