Structural and molecular remodeling of dendritic spine substructures during long-term potentiation.
Structural and molecular remodeling of dendritic spine substructures during long-term potentiation.
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DOI：
10.1016/j.neuron.2014.03.021
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发表时间：
2014-04-16
期刊：
影响因子：
16.2
通讯作者：
Hayashi Y
中科院分区：
文献类型：
--
作者：
Bosch M;Castro J;Saneyoshi T;Matsuno H;Sur M;Hayashi Y
突触通过其结构和分子组成的长期修改来存储信息，但这些变化的精确时间表尚未在真实的时间内以单个突触分辨率进行研究。在这里，我们描述了在长时程增强（LTP）在个别树突棘突触后亚结构的时空重组。蛋白质通过三个连续的阶段以四种不同的模式转移到脊柱。在初始阶段，肌动蛋白细胞骨架迅速重塑，而活性cofilin被大量运输到脊柱。在稳定阶段，cofilin与F-肌动蛋白形成稳定的复合物，持续保留在脊柱处，并巩固脊柱扩张。相反，突触后密度（PSD）独立重塑，PSD支架蛋白没有改变其数量和位置，直到后期蛋白质合成依赖的第三阶段。我们的研究结果显示了如何以及何时在LTP过程中重建脊柱亚结构，并解释了为什么突触可塑性规则随时间而变化。
Synapses store information by long-lasting modifications of their structure and molecular composition, but the precise chronology of these changes has not been studied at single synapse resolution in real time. Here we describe the spatiotemporal reorganization of postsynaptic substructures during long-term potentiation (LTP) at individual dendritic spines. Proteins translocated to the spine in four distinct patterns through three sequential phases. In the initial phase, the actin cytoskeleton was rapidly remodeled while active cofilin was massively transported to the spine. In the stabilization phase, cofilin formed a stable complex with F-actin, was persistently retained at the spine, and consolidated spine expansion. In contrast, the postsynaptic density (PSD) was independently remodeled, as PSD scaffolding proteins did not change their amount and localization until a late protein synthesis-dependent third phase. Our findings show how and when spine substructures are remodeled during LTP and explain why synaptic plasticity rules change over time.
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影响因子：
25
作者：
Gu, Jiaping;Lee, Chi Wai;Fan, Yanjie;Komlos, Daniel;Tang, Xin;Sun, Chicheng;Yu, Kuai;Hartzell, H. Criss;Chen, Gong;Bamburg, James R.;Zheng, James Q.
通讯作者：
Zheng, James Q.
影响因子：
64.8
作者：
Lee, Seok-Jin R.;Escobedo-Lozoya, Yasmin;Szatmari, Erzsebet M.;Yasuda, Ryohei
通讯作者：
Yasuda, Ryohei
DOI：
10.1523/jneurosci.1746-10.2010
发表时间：
2010-09-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Fortin DA;Davare MA;Srivastava T;Brady JD;Nygaard S;Derkach VA;Soderling TR
通讯作者：
Soderling TR
影响因子：
16.2
作者：
Frost, Nicholas A.;Shroff, Hari;Kong, Huihui;Betzig, Eric;Blanpied, Thomas A.
通讯作者：
Blanpied, Thomas A.
影响因子：
16
作者：
Andrianantoandro, Ernesto;Pollard, Thomas D.
通讯作者：
Pollard, Thomas D.