Cellular Patterning of Arabidopsis Roots Under Low Phosphate Conditions.
Cellular Patterning of Arabidopsis Roots Under Low Phosphate Conditions.
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DOI：
10.3389/fpls.2018.00735
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发表时间：
2018
影响因子：
5.6
通讯作者：
Bishopp A
中科院分区：
文献类型：
--
作者：
Janes G;von Wangenheim D;Cowling S;Kerr I;Band L;French AP;Bishopp A
磷是一种重要的植物常量营养素，在许多细胞信号传导和能量循环过程中起着关键作用。因此，磷获取效率是作物改良的重要目标性状，同时也为植物在低营养环境下的生长提供了生态适应性。在许多物种中，增加根毛密度已被证明可以改善磷的吸收和植物健康。在包括芸苔科在内的几个植物科中，根生毛细胞根据其相对于皮层细胞的位置位于表皮上，毛细胞位于两个皮层细胞之间的间隙中。因此，皮层细胞的数量决定了根毛位置表皮细胞的数量。先前的研究已经将磷限制条件与拟南芥根皮层细胞档案数量的增加联系起来，但他们没有调查这些额外分裂发生的空间或时间域，也没有探索这对根毛形成的影响。在这项研究中，我们使用根分生组织的三维重建来证明，在低磷酸盐条件下，径向背斜细胞分裂只发生在皮层。当在含磷充足的培养基上生长时，拟南芥几乎无一例外地显示出8个皮质细胞；然而，当在磷酸盐限制条件下生长时，幼苗发育到16个皮质细胞（最典型的是10-14个）。这导致毛发形成部位表皮细胞数量显著增加。这些径向背斜分裂发生在初始细胞内，并在植物转移到低磷条件的24小时内可见。我们发现这些皮层细胞的变化通过改变根毛的规则间距而进入表皮模式。
Phosphorus is a crucial macronutrient for plants playing a critical role in many cellular signaling and energy cycling processes. In light of this, phosphorus acquisition efficiency is an important target trait for crop improvement, but it also provides an ecological adaptation for growth of plants in low nutrient environments. Increased root hair density has been shown to improve phosphorus uptake and plant health in a number of species. In several plant families, including Brassicaceae, root hair bearing cells are positioned on the epidermis according to their position in relation to cortex cells, with hair cells positioned in the cleft between two underlying cortex cells. Thus the number of cortex cells determines the number of epidermal cells in the root hair position. Previous research has associated phosphorus-limiting conditions with an increase in the number of cortex cell files in Arabidopsis thaliana roots, but they have not investigated the spatial or temporal domains in which these extra divisions occur or explored the consequences this has had on root hair formation. In this study, we use 3D reconstructions of root meristems to demonstrate that the radial anticlinal cell divisions seen under low phosphate are exclusive to the cortex. When grown on media containing replete levels of phosphorous, A. thaliana plants almost invariably show eight cortex cells; however when grown in phosphate limited conditions, seedlings develop up to 16 cortex cells (with 10–14 being the most typical). This results in a significant increase in the number of epidermal cells at hair forming positions. These radial anticlinal divisions occur within the initial cells and can be seen within 24 h of transfer of plants to low phosphorous conditions. We show that these changes in the underlying cortical cells feed into epidermal patterning by altering the regular spacing of root hairs.
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影响因子：
16.6
作者：
Bhosale R;Giri J;Pandey BK;Giehl RFH;Hartmann A;Traini R;Truskina J;Leftley N;Hanlon M;Swarup K;Rashed A;Voß U;Alonso J;Stepanova A;Yun J;Ljung K;Brown KM;Lynch JP;Dolan L;Vernoux T;Bishopp A;Wells D;von Wirén N;Bennett MJ;Swarup R
通讯作者：
Swarup R
影响因子：
4.6
作者：
Hassan, Hala;Scheres, Ben;Blilou, Ikram
通讯作者：
Blilou, Ikram
影响因子：
7.3
作者：
Ma, Z;Bielenberg, DG;Lynch, JP
通讯作者：
Lynch, JP
影响因子：
4.2
作者：
Pemberton, LMS;Tsai, SL;Harris, PJ
通讯作者：
Harris, PJ
影响因子：
4.6
作者：
Bernhardt, C;Lee, MM;Schiefelbein, J
通讯作者：
Schiefelbein, J