Oral vaccination with LcrV from Yersinia pestis KIM delivered by live attenuated Salmonella enterica serovar Typhimurium elicits a protective immune response against challenge with Yersinia pseudotuberculosis and Yersinia enterocolitica.
Oral vaccination with LcrV from Yersinia pestis KIM delivered by live attenuated Salmonella enterica serovar Typhimurium elicits a protective immune response against challenge with Yersinia pseudotuberculosis and Yersinia enterocolitica.
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DOI：
10.1016/j.vaccine.2009.06.078
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发表时间：
2009-08-27
期刊：
影响因子：
5.5
通讯作者：
Curtiss R 3rd
中科院分区：
文献类型：
--
作者：
Branger CG;Torres-Escobar A;Sun W;Perry R;Fetherston J;Roland KL;Curtiss R 3rd
使用合成耶尔森氏菌蛋白的活重组减毒沙门氏菌疫苗（RASV）是控制耶尔森氏菌属物种感染的有希望的方法。在这项研究中，我们构建了减毒沙门氏菌菌株，合成的截短形式的LcrV，LcrV196和评估的免疫反应和保护效力引起的这些菌株在小鼠对其他两个主要物种的耶尔森氏菌：假结核耶尔森氏菌和小肠结肠炎耶尔森氏菌。令人惊讶的是，我们发现单独的RASV菌株足以提供几乎完全的保护以抵抗Y的攻击。假结核，表明沙门氏菌产生免疫原性交叉保护抗原的可能性。相反，lcrV196表达是针对用Y.小肠结肠炎由于LcrV先前已显示可提供针对鼠疫耶尔森氏菌的保护，因此这些发现表明单一疫苗可提供针对耶尔森氏菌的三种人类致病性物种的保护。
The use of live recombinant attenuated Salmonella vaccines (RASV) synthesizing Yersinia proteins is a promising approach for controlling infection by Yersinia species. In this study, we constructed attenuated Salmonella strains which synthesize a truncated form of LcrV, LcrV196 and evaluated the immune response and protective efficacy elicited by these strains in mice against two other major species of Yersinia: Yersinia pseudotuberculosis and Yersinia enterocolitica. Surprisingly, we found that the RASV strain alone was sufficient to afford nearly full protection against challenge with Y. pseudotuberculosis, indicating the likelihood that Salmonella produces immunogenic cross-protective antigens. In contrast, lcrV196 expression was required for protection against challenge with Y. enterocolitica. As LcrV has been previously shown to provide protection against Yersinia pestis, these findings indicate that a single vaccine may provide protection against the three human pathogenic species of Yersinia.
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影响因子：
--
作者：
Kang, HY;Curtiss, R
通讯作者：
Curtiss, R
影响因子：
3.1
作者：
Maier, RJ;Olczak, A;Gunn, J
通讯作者：
Gunn, J
影响因子：
24.5
作者：
GRUTZKAU, A;HANSKI, C;RIECKEN, EO
通讯作者：
RIECKEN, EO
影响因子：
64.5
作者：
KOSHLAND, D;BOTSTEIN, D
通讯作者：
BOTSTEIN, D
DOI：
10.1073/pnas.0507749103
发表时间：
2006-02-28
影响因子：
11.1
作者：
Heise, T;Dersch, P
通讯作者：
Dersch, P