Distinct Oceanic Microbiomes From Viruses to Protists Located Near the Antarctic Circumpolar Current.
Distinct Oceanic Microbiomes From Viruses to Protists Located Near the Antarctic Circumpolar Current.
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DOI：
10.3389/fmicb.2018.01474
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发表时间：
2018
影响因子：
5.2
通讯作者：
Rybicki EP
中科院分区：
文献类型：
--
作者：
Flaviani F;Schroeder DC;Lebret K;Balestreri C;Highfield AC;Schroeder JL;Thorpe SE;Moore K;Pasckiewicz K;Pfaff MC;Rybicki EP
微生物占据着不同的生态位，只有通过新一代测序技术的最新进展，才能揭示真正的微生物多样性。此外，由于缺乏可察觉的阻止遗传扩散的海洋障碍(即山脉或岛屿)，人们得以猜测，生物可以很容易地被洋流输送，因此到处都在繁殖。也就是说，洋流现在被普遍认为是微生物扩散的障碍。在这里，我们分析了从总共六个站点收集的样本，其中四个位于印度洋，两个位于南大洋。扩增子测序用于描述原核和真核浮游生物群落，而鸟枪测序用于环境DNA(Edna)、微生物edna(MeDNA)和病毒组分的组合。我们发现，蓝藻在西南印度洋的原核成分中占主导地位，而γ-变形杆菌在东南印度洋中占主导地位。γ-和α-变形杆菌的组合主宰了南大洋。泡状目几乎完全是真核生物的组成部分，原泡目和鞭毛目的比例随站位的不同而不同。然而，在南大洋观察到了触生植物相对丰度的增加。同样，在所有站点中，病毒部分主要是尾状病毒目的成员；然而，在南大洋观察到更多的核质大DNA病毒(主要是氯病毒和Mimi病毒)。据我们所知，这是第一次在亚热带印度洋和南大洋之间的微生物群(从病毒到原生生物)的统计差异。我们还表明，并不是所有的系统类型都可以随处可见，meDNA也不是监测水生微生物多样性的合适资源。
Microbes occupy diverse ecological niches and only through recent advances in next generation sequencing technologies have the true microbial diversity been revealed. Furthermore, lack of perceivable marine barriers to genetic dispersal (i.e., mountains or islands) has allowed the speculation that organisms that can be easily transported by currents and therefore proliferate everywhere. That said, ocean currents are now commonly being recognized as barriers for microbial dispersal. Here we analyzed samples collected from a total of six stations, four located in the Indian Ocean, and two in the Southern Ocean. Amplicon sequencing was used to characterize both prokaryotic and eukaryotic plankton communities, while shotgun sequencing was used for the combined environmental DNA (eDNA), microbial eDNA (meDNA), and viral fractions. We found that Cyanobacteria dominated the prokaryotic component in the South-West Indian Ocean, while γ-Proteobacteria dominated the South-East Indian Ocean. A combination of γ- and α-Proteobacteria dominated the Southern Ocean. Alveolates dominated almost exclusively the eukaryotic component, with variation in the ratio of Protoalveolata and Dinoflagellata depending on station. However, an increase in haptophyte relative abundance was observed in the Southern Ocean. Similarly, the viral fraction was dominated by members of the order Caudovirales across all stations; however, a higher presence of nucleocytoplasmic large DNA viruses (mainly chloroviruses and mimiviruses) was observed in the Southern Ocean. To our knowledge, this is the first that a statistical difference in the microbiome (from viruses to protists) between the subtropical Indian and Southern Oceans. We also show that not all phylotypes can be found everywhere, and that meDNA is not a suitable resource for monitoring aquatic microbial diversity.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.4
作者：
Claverie, Jean-Michel;Grzela, Renata;Abergel, Chantal
通讯作者：
Abergel, Chantal
影响因子：
5.7
作者：
Dejean, Tony;Valentini, Alice;Miaud, Claude
通讯作者：
Miaud, Claude
影响因子：
13.5
作者：
Donlon, Craig J.;Martin, Matthew;Wimmer, Werenfrid
通讯作者：
Wimmer, Werenfrid
影响因子：
6.1
作者：
de Roos, AM;Leonardsson, K;Mittelbach, GG
通讯作者：
Mittelbach, GG