rab4 Regulates Transport to the Apical Plasma Membrane in Madin-Darby Canine Kidney Cells*
rab4 Regulates Transport to the Apical Plasma Membrane in Madin-Darby Canine Kidney Cells*
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rab4 调节 Madin-Darby 犬肾细胞向顶端质膜的运输*
DOI：
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发表时间：
2002
影响因子：
4.8
通讯作者：
P. van der Sluijs
中科院分区：
文献类型：
--
作者：
K. Mohrmann;R. Leijendekker;L. Gerez;P. van der Sluijs
小的GTP酶Rab4与早期的内小体有关，并调节成纤维细胞的膜循环。Rab4存在于上皮细胞中，但其在这类细胞中的定位和功能尚未确定。我们用Rab4、GTP酶缺陷突变体Rab4Q67L和显性负性突变体Rab4S22N(与鸟嘌呤核苷酸结合不良)导入Madin-Darby犬肾细胞。共聚焦免疫荧光显微镜显示，Rab4主要集中在细胞核周围细胞外侧的内部结构中。定量免疫电子显微镜显示，Rab4主要定位于细胞质的上1/3。在与125I-转铁蛋白的细胞表面结合实验中，我们发现在表达Rab4和Rab4Q67L的细胞中，转铁蛋白受体从顶端质膜的基底外侧重新分布。在16 °C时，转铁蛋白在基底外侧早期内吞体内积聚后，Rab4和Rab4Q67L增加了顶端靶向转铁蛋白受体的数量。灯盏花素A和Rab4对转铁蛋白受体顶端靶向的作用不是相加的，提示灯盏花素A和Rab4可能作用于共同内体的相同转运途径。
The small GTPase rab4 is associated with early endosomes and regulates membrane recycling in fibroblasts. rab4 is present in epithelial cells; however, neither its localization nor function has been established in this cell type. We transfected Madin-Darby canine kidney cells with rab4, the GTPase-deficient mutant rab4Q67L, and the dominant negative mutant rab4S22N that poorly binds guanine nucleotides. Confocal immunofluorescence microscopy showed that rab4 was concentrated on internal structures at the lateral side of the cell around the nucleus. Quantitative immunoelectron microscopy revealed that the majority of rab4 was localized in the upper third of the cytoplasm. In cell surface binding experiments with125I-transferrin, we found a redistribution of transferrin receptor from the basolateral to the apical plasma membrane in cells expressing rab4 and rab4Q67L. After accumulation of transferrin at 16 °C in basolateral early endosomes, rab4 and rab4Q67L increased the amount of apically targeted transferrin receptor. A qualitatively similar effect was obtained in control cells treated with brefeldin A. The effects of brefeldin A and rab4 on apical targeting of transferrin receptor were not additive, suggesting that brefeldin A and rab4 may act in the same transport pathway from common endosomes.
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影响因子：
3.3
作者：
Wilson, JM;de Hoop, M;Parton, RG
通讯作者：
Parton, RG
来自大鼠肝脏的管状内体部分：默认情况下受体分选的生化证据。
DOI：
10.1073/pnas.96.18.10146
发表时间：
1999
影响因子：
11.1
作者：
Vergés,M;Havel,RJ;Mostov,KE
通讯作者：
Mostov,KE
DOI：
10.1006/meth.1998.0662
发表时间：
1998-10-01
期刊：
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY
影响因子：
--
作者：
Clift-O'Grady, L;Desnos, C;Kelly, RB
通讯作者：
Kelly, RB
影响因子：
3.3
作者：
de Wit, H;Lichtenstein, Y;van der Sluijs, P
通讯作者：
van der Sluijs, P
影响因子：
3.3
作者：
Casanova, JE;Wang, XY;Goldenring, JR
通讯作者：
Goldenring, JR