Functional repertoire convergence of distantly related eukaryotic plankton lineages abundant in the sunlit ocean.
Functional repertoire convergence of distantly related eukaryotic plankton lineages abundant in the sunlit ocean.
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DOI：
10.1016/j.xgen.2022.100123
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发表时间：
2022-05-11
期刊：
影响因子：
--
通讯作者：
Jaillon, Olivier
中科院分区：
文献类型：
--
作者：
Delmont, Tom O.;Gaia, Morgan;Hinsinger, Damien D.;Fremont, Paul;Vanni, Chiara;Fernandez-Guerra, Antonio;Eren, A. Murat;Kourlaiev, Artem;d'Agata, Leo;Clayssen, Quentin;Villar, Emilie;Labadie, Karine;Cruaud, Corinne;Poulain, Julie;Da Silva, Corinne;Wessner, Marc;Noel, Benjamin;Aury, Jean-Marc;de Vargas, Colomban;Bowler, Chris;Karsenti, Eric;Pelletier, Eric;Wincker, Patrick;Jaillon, Olivier
海洋浮游真核生物在全球生物地球化学循环和气候中发挥着重要作用。然而，它们在文化收藏中的代表性不足，限制了我们对浮游生态系统的进化史和基因组基础的理解。在这里，我们使用了来自极地、温带和热带阳光照射海洋的2800亿Tara海洋元基因组读数，重建和人工整理了700多个丰富而广泛的真核生物环境基因组，范围从10兆bp到1.3兆bp不等。这种基因组资源涵盖了广泛的特征不明确的真核生物谱系，补充了长期以来来自培养集合的贡献，同时更好地代表了海洋上层的浮游生物。据我们所知，我们首次对丰富的单细胞真核浮游生物进行了全面的全基因组功能分类，揭示了四个主要的类群，这些类群连接着远亲谱系。浮游生物的营养模式及其垂直进化史都不能完全解释数百万年来在洋流中共存的主要真核生物谱系的功能集合。研究人员对海洋中浮游生物的近3000亿个宏基因组进行了共组装，并对数百个真核生物环境基因组进行了表征和整理。这些基因组与人工培养相比，更好地代表了真核生物。这些基因组揭示了远亲真核生物的功能聚合。Delmont等人利用近3000亿个宏基因组来表征阳光照射下海洋中一些最丰富和最广泛的真核生物种群的基因组内容。这个庞大的基因组资源涵盖了我们的文化组合中代表性不足的分类群，并揭示了远亲真核浮游生物谱系的功能趋同。
Marine planktonic eukaryotes play critical roles in global biogeochemical cycles and climate. However, their poor representation in culture collections limits our understanding of the evolutionary history and genomic underpinnings of planktonic ecosystems. Here, we used 280 billion Tara Oceans metagenomic reads from polar, temperate, and tropical sunlit oceans to reconstruct and manually curate more than 700 abundant and widespread eukaryotic environmental genomes ranging from 10 Mbp to 1.3 Gbp. This genomic resource covers a wide range of poorly characterized eukaryotic lineages that complement long-standing contributions from culture collections while better representing plankton in the upper layer of the oceans. We performed the first, to our knowledge, comprehensive genome-wide functional classification of abundant unicellular eukaryotic plankton, revealing four major groups connecting distantly related lineages. Neither trophic modes of plankton nor its vertical evolutionary history could completely explain the functional repertoire convergence of major eukaryotic lineages that coexisted within oceanic currents for millions of years. Nearly 300 billion metagenomic reads were co-assembled from plankton in the oceans Hundreds of eukaryotic environmental genomes were characterized and curated These genomes better represent eukaryotic plankton compared to cultivation These genomes reveal a functional convergence of distantly related eukaryotes Delmont et al. use nearly 300 billion metagenomic reads to characterize the genomic content of some of the most abundant and widespread eukaryotic populations in the sunlit ocean. This large genomic resource covers taxa underrepresented in our culture portfolio and exposes a functional convergence of distantly related eukaryotic plankton lineages.
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影响因子：
2.7
作者：
Delmont TO;Eren AM
通讯作者：
Eren AM
影响因子：
12.3
作者：
Denoeud F;Aury JM;Da Silva C;Noel B;Rogier O;Delledonne M;Morgante M;Valle G;Wincker P;Scarpelli C;Jaillon O;Artiguenave F
通讯作者：
Artiguenave F
影响因子：
3.7
作者：
Boyd, Philip W.
通讯作者：
Boyd, Philip W.
影响因子：
4.5
作者：
Costa, Raul Rodrigo;Mendes, Carlos Rafael Borges;Secchi, Eduardo Resende
通讯作者：
Secchi, Eduardo Resende
影响因子：
5.2
作者：
Delmont TO;Eren AM;Vineis JH;Post AF
通讯作者：
Post AF