Studies of the Role of the Propeptides of the Arabidopsis thaliana 2S Albumin
Studies of the Role of the Propeptides of the Arabidopsis thaliana 2S Albumin
复制标题
拟南芥2S白蛋白前肽作用的研究
DOI：
10.1104/pp.102.2.425
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发表时间：
1993
影响因子：
6.1
通讯作者：
E. Krebbers
中科院分区：
文献类型：
--
作者：
K. D'hondt;J. Van Damme;C. Van den Bossche;S. Leejeerajumnean;R. De Rycke;J. Derksen;J. Vandekerckhove;E. Krebbers
为了研究拟南芥2S白蛋白前肽在植物细胞中对蛋白质的分选、加工和稳定性的可能作用，根据拟南芥2S白蛋白编码基因之一，构建了5个删除或修饰这些前肽的基因。这些结构被导入烟草(Nicitiana Tabacum)植株。对成熟种子进行亚细胞分离和免疫细胞化学实验，结果表明，蛋白质的分离不需要任何一种前肽。对成熟基因产物的详细蛋白质化学分析表明，所有修饰的2S白蛋白前体蛋白都能稳定折叠并正确加工。然而，当小亚基和大亚基之间的内部片段丢失或该内部片段改变时，后者的效率较低。为了建立修饰的2S白蛋白前体的快速检测体系，用相同的基因构建物转化酵母细胞。这表明酵母细胞中的加工机制与植物中的不同，在可能相关的观察中，观察到了特定修饰蛋白质的稳定性的差异。
To investigate the possible roles of the Arabidopsis thaliana 2S albumin propeptides with respect to sorting, processing, and stability of the protein in plant cells, five gene constructions deleting or modifying the propeptides were made based on one of the genes encoding the Arabidopsis 2S albumin. These constructions were introduced into tobacco (Nicotiana tabacum) plants. Using subcellular fractionation and immunocytochemistry on ripe seeds, it was demonstrated that none of the propeptides was necessary for the sorting of the protein. Detailed protein-chemical analysis of the mature gene products indicated that, for all of the modified 2S albumin precursors made, the proteins were stably folded and correctly processed. However, the latter is less efficient when the internal fragment between the small and the large subunit is missing or when this internal fragment is changed. In an attempt to establish a rapid assay system for modified 2S albumin precursors, yeast cells were transformed with the same gene constructs. It was demonstrated that the processing machinery in yeast cells differs from that in plants, and, in a perhaps related observation, differences in stability of a particular modified protein were observed.
影响因子：
3.5
作者：
KLEBE, RJ;HARRISS, JV;DOUGLAS, MG
通讯作者：
DOUGLAS, MG