Diagnostic Utility of HPV16 E6 mRNA or E7 mRNA Quantitative Expression for Cervical Cells of Patients with Dysplasia and Carcinoma.
Diagnostic Utility of HPV16 E6 mRNA or E7 mRNA Quantitative Expression for Cervical Cells of Patients with Dysplasia and Carcinoma.
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HPV16 E6 mRNA 或 E7 mRNA 定量表达对不典型增生和癌患者宫颈细胞的诊断价值
DOI：
10.1177/0963689718788521
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发表时间：
2018-09
影响因子：
3.3
通讯作者：
Wu GP
中科院分区：
文献类型：
--
作者：
Wu MZ;Li WN;Cha N;Tian LX;Zhang YI;Wu X;Guo KJ;Wu GP
目前人乳头瘤病毒(HPV)16DNA检测具有较高的敏感性，但特异性较低，而mRNA检测(定性)提高了特异性。然而，这两种技术都不能区分一过性感染和持续性感染。为了克服这些缺点，我们用实时定量聚合酶链式反应定量检测了87例HPV16+和31例HPV16型−患者宫颈刷检细胞中的E6和E7mRNAs。结果表明，HPV16阳性病例中E6、E7mRNA的表达水平明显高于HPV16阴性病例。此外，在HPV16+病例中，浸润性癌中E6mRNA的表达水平显著高于高度鳞状上皮内病变(HSIL；p<；0.01)和HSIL与低度鳞状上皮内病变(Lsil；p<；0.01)。LSIL与良性病变之间无明显差异。E7mRNA的表达水平在上述四组间无显著差异。为了验证qRT-PCR能否区分一过性感染和持续性感染，我们对57例HPV16+患者进行了为期一年的随访，结果显示持续感染组E6和E7mRNA的表达水平均显著高于一过性感染组(p<；0.01或0.05)。因此，定量检测宫颈刷检细胞中E6mRNA的表达水平，不仅可以作为宫颈肿瘤细胞学诊断的辅助工具，而且可能有助于确定病变的严重程度和一过性感染的分类。
Current human papillomavirus (HPV)16 DNA testing has high sensitivity but low specificity, while mRNA testing (qualitative) improves the specificity. However, both techniques are not able to discriminate between transient and persistent infections. To overcome the disadvantages, we quantitatively detected E6 and E7 mRNAs by quantitative real-time polymerase chain reaction (qRT-PCR) in cervical brushing cells from 87 HPV16+ and 31 HPV16− patients. Our results showed that the expression levels of E6 mRNA or E7 mRNA were significantly increased in HPV16-positive cases than that in the negative cases. Furthermore, in HPV16+ cases, the expression levels of E6 mRNA were significantly increased in invasive cancer compared with high-grade squamous intraepithelial lesion (HSIL; p < 0.01), and HSIL compared with low-grade squamous intraepithelial lesion (LSIL; p < 0.01). There were no significant changes between LSIL and benign lesions. The expression levels of E7 mRNA presented no significant difference among the above-mentioned four groups. To test whether qRT-PCR can discriminate between transient and persistent infections, 57 HPV16+ patients were followed up for 1 year, and our results demonstrated that the expression levels of both E6 mRNA and E7 mRNA in the persistent infection group were significantly increased relative to the transient infection group (p < 0.01 or 0.05). Thus, a quantitative detection of the expression levels of E6 mRNA in cervical brushing cells may not only be used as an ancillary tool to cytological diagnosis of cervical neoplasia, but may also help to determine the severity of the lesions and the triage of transient infection.
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影响因子：
168.9
作者：
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