Determinants of intramyocellular lipid accumulation after dietary fat loading in non-obese men.
Determinants of intramyocellular lipid accumulation after dietary fat loading in non-obese men.
复制标题
DOI：
10.1111/j.2040-1124.2010.00091.x
复制
发表时间：
2011-08-02
影响因子：
3.2
通讯作者：
Watada H
中科院分区：
文献类型：
--
作者：
Sakurai Y;Tamura Y;Takeno K;Kumashiro N;Sato F;Kakehi S;Ikeda S;Ogura Y;Saga N;Naito H;Katamoto S;Fujitani Y;Hirose T;Kawamori R;Watada H
目的/简介：细胞内脂质（IMCL）的积累与胰岛素抵抗有关。然而，影响IMCL变化的因素仍有待阐明。本研究的目的是确定影响高脂负荷后IMCL水平变化的因素。材料和方法：研究对象为37名非肥胖男性。每名受试者在正常脂肪饮食3天后食用高脂肪饮食3天。每个饮食计划结束后，用质子磁共振波谱法测量胫骨前肌（TA‐IMCL）和比目鱼肌（SOL‐IMCL）的IMCL水平。葡萄糖输注率（GIR）采用正糖高胰岛素钳作为外周胰岛素敏感性指标。结果：高脂饮食显著提高了TA - IMCL和SOL - IMCL，分别提高了~ 30%和~ 20% (P < 0.05)，而GIR没有显著改变。SOL‐IMCL升高与血清高分子量（HMW）‐脂联素（r = - 0.36, P < 0.05）和HMW‐/总脂联素比值（r = - 0.46, P < 0.05）呈负相关，而TA‐IMCL无升高。虽然高脂饮食相关的SOL - IMCL变化表现出较高的个体间差异，但在进行运动的受试者中，SOL - IMCL （r = 0.55, P < 0.05）和TA - IMCL （r = 0.61, P < 0.05）的变化与日常体力活动水平呈正相关。相反，在久坐的受试者中，SOL‐IMCL （r = - 0.50, P < 0.01）和TA‐IMCL （r = - 0.48, P < 0.05）的变化与日常体力活动呈负相关。结论：HMW -脂联素和日常体力活动是高脂饮食中IMCL积累的决定因素。有趣的是，日常体育活动对IMCL变化的影响取决于定期锻炼的水平。[J] .糖尿病投资杂志，doi: 10.1111/ J .2040‐1124.2010.00091。x, 2011)
Aims/Introduction: Accumulation of intramyocellular lipid (IMCL) is associated with insulin resistance. However, the factors affecting the change in IMCL remain to be elucidated. The aim of the present study was to determine the factors that influence the change in IMCL level after high‐fat loading. Materials and Methods: The study subjects were 37 non‐obese men. Each subject consumed a high‐fat diet for 3 days after a normal‐fat diet for 3 days. After each diet program, IMCL levels in the tibialis anterior (TA‐IMCL) and soleus (SOL‐IMCL) were measured by proton magnetic resonance spectroscopy. Glucose infusion rate (GIR) was evaluated by euglycemic hyperinsulinemic clamp as an index of peripheral insulin sensitivity. Results: The high‐fat diet significantly increased TA‐IMCL and SOL‐IMCL by ∼30 and ∼20%, respectively (P < 0.05), whereas it did not significantly alter GIR. The increase in SOL‐IMCL, but not in TA‐IMCL, negatively correlated with serum high molecular weight (HMW)‐adiponectin (r = −0.36, P < 0.05) and HMW‐/total‐adiponectin ratio (r = −0.46, P < 0.05). Although high‐fat diet‐related changes in SOL‐IMCL showed high inter‐individual variations, in subjects doing exercise, changes in SOL‐IMCL (r = 0.55, P < 0.05) and TA‐IMCL (r = 0.61, P < 0.05) positively correlated with daily physical activity level. In contrast, in sedentary subjects, changes in SOL‐IMCL (r = −0.50, P < 0.01) and TA‐IMCL (r = −0.48, P < 0.05) negatively correlated with daily physical activity. Conclusions: HMW‐adiponectin and daily physical activity are determinants of IMCL accumulation by a high‐fat diet. Intriguingly, the effect of daily physical activity on the change in IMCL depends on the level of regular exercise. (J Diabetes Invest,doi: 10.1111/j.2040‐1124.2010.00091.x, 2011)
登录
查看更多内容
影响因子：
8.2
作者：
Krssak, M;Petersen, KF;Roden, M
通讯作者：
Roden, M
影响因子：
5.8
作者：
Goodpaster, BH;He, J;Kelley, DE
通讯作者：
Kelley, DE
影响因子：
7.7
作者：
Kelley, DE;Mandarino, LJ
通讯作者：
Mandarino, LJ
影响因子：
5.8
作者：
Tamura, Y.;Watada, H.;Kawamori, R.
通讯作者：
Kawamori, R.
影响因子：
4.8
作者：
Pajvani, UB;Hawkins, M;Scherer, PE
通讯作者：
Scherer, PE