Development of an immunosensor for the determination of allergy antibody (IgE) in blood samples
Development of an immunosensor for the determination of allergy antibody (IgE) in blood samples
复制标题
开发用于测定血液样本中过敏抗体 (IgE) 的免疫传感器
DOI：
10.1016/s0003-2670(01)01137-0
复制
发表时间：
2001
影响因子：
6.2
通讯作者：
G. Guilbault
中科院分区：
文献类型：
--
作者：
M. Kreuzer;C. O’Sullivan;M. Pravda;G. Guilbault
目前的工作描述了使用一次性丝网印刷碳电极技术优化血液样本中过敏抗体（IgE）的测量系统，并在+300mV与Ag/AgCl下对碱性磷酸酶产生的对氨基酚进行安培检测。该系统首先通过酶联免疫吸附试验（ELISA）开发，然后通过丝网印刷电极进行分析。该传感器结合了敏感患者的临床相关IgE范围，检测限为0.09ngml - 1IgE。系统简单，只需要添加分析物（样品），分析在30分钟内完成。此外，它具有成本效益，并且被开发用作护理点设备，能够测量一滴血中的IgE水平。
The current work describes the optimisation of a system for measurement of allergy antibodies (IgE) in blood samples using disposable screen-printed carbon electrode technology and amperometric detection of p-aminophenol, produced by alkaline phosphatase, at +300mV versus Ag/AgCl. The system was primarily developed by enzyme-linked immunosorbent assay (ELISA) prior to analysis by screen-printed electrodes. The sensor incorporates the clinically relevant range of IgE in sensitised patients with a detection limit of 0.09ngml−1IgE. The system is simple, requiring only addition of analyte (sample) and analysis is complete in 30min. In addition, it is cost effective and was developed for use as a point of care device, capable of measuring IgE levels in a drop of blood.
影响因子：
7.4
作者：
C. Y. Tu;T. Kitamori;T. Sawada;H. Kimura;S. Matsuzawa
通讯作者：
C. Y. Tu;T. Kitamori;T. Sawada;H. Kimura;S. Matsuzawa