Activation of human monocytes after infection by human coronavirus 229E.
Activation of human monocytes after infection by human coronavirus 229E.
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DOI：
10.1016/j.virusres.2007.06.016
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发表时间：
2007-12
期刊：
影响因子：
5
通讯作者：
Talbot PJ
中科院分区：
文献类型：
--
作者：
Desforges M;Miletti TC;Gagnon M;Talbot PJ
人冠状病毒（HCoV）是公认的呼吸道病原体，其可能涉及其他病理，如中枢神经系统（CNS）疾病。为了研究白细胞是否可以参与呼吸道病理学并作为病毒向其他组织传播的载体，研究了人白细胞细胞系和外周血单核细胞（PBMC）对HCoV-229 E和HCoV-OC 43感染的易感性。人原代单核细胞/巨噬细胞对HCoV-229 E感染敏感，但强烈限制HCoV-OC 43复制。此外，原代单核细胞和THP-1单核细胞系的生产性HCoV-229 E感染导致其活化，如促炎介质（包括TNF-α、CCL 5、CXCL 10和CXCL 11以及MMP-9）的产生所示。此外，体外趋化性测定显示，在急性或持续性HCoV-229 E感染后，THP-1细胞和原代单核细胞朝向趋化因子的运动性增加。总之，这些结果表明，感染的单核细胞可以作为HCoV-229 E的储存库，被激活，参与肺部病理的恶化，以及作为病毒传播到宿主组织的潜在载体，在那里它可能与其他病理相关。
Human coronaviruses (HCoV) are recognized respiratory pathogens that may be involved in other pathologies such as central nervous system (CNS) diseases. To investigate whether leukocytes could participate in respiratory pathologies and serve as vector for viral spread towards other tissues, the susceptibility of human leukocytic cell lines and peripheral blood mononuclear cells (PBMC) to HCoV-229E and HCoV-OC43 infection was investigated. Human primary monocytes/macrophages were susceptible to HCoV-229E infection, but strongly restricted HCoV-OC43 replication. Moreover, productive HCoV-229E infection of primary monocytes and of the THP-1 monocytic cell line led to their activation, as indicated by the production of pro-inflammatory mediators, including TNF-α, CCL5, CXCL10 and CXCL11 and MMP-9. Moreover, an in vitro chemotaxis assay showed that motility towards chemokines of THP-1 cells and primary monocytes was increased following an acute or persistent HCoV-229E infection. Taken together, these results suggest that infected monocytes could serve as a reservoir for HCoV-229E, become activated, participate in the exacerbation of pulmonary pathologies, as well as serve as potential vectors for viral dissemination to host tissues, where it could be associated with other pathologies.
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