A proteome map of primary cultured rat Schwann cells.
A proteome map of primary cultured rat Schwann cells.
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原代培养的大鼠雪旺细胞的蛋白质组图谱
DOI：
10.1186/1477-5956-10-20
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发表时间：
2012-03-23
期刊：
影响因子：
2
通讯作者：
Ding F
中科院分区：
文献类型：
--
作者：
Shen M;Ji Y;Zhang S;Shi H;Chen G;Gu X;Ding F
雪旺细胞（SCs）是周围神经系统的主要胶质细胞，具有广泛的生物学功能。SCs在周围神经再生中起关键作用，并参与多种遗传性周围神经病变。本研究的目的是对SCs的全蛋白组成有新的认识。结果采用二维液相色谱-串联质谱法（2D LC-MS/MS）鉴定大鼠原代培养SCs中蛋白的表达。我们共鉴定出1232种蛋白质，将其分为20个功能类。我们还使用实时定量RT-PCR和Western blot分析来验证一些蛋白质组学鉴定的蛋白质。结论首次获得了SCs的蛋白质组图谱。我们的数据可以作为一个参考库，为了解SC生物学提供基础信息。
BackgroundSchwann cells (SCs) are the principal glial cells of the peripheral nervous system with a wide range of biological functions. SCs play a key role in peripheral nerve regeneration and are involved in several hereditary peripheral neuropathies. The objective of this study was to gain new insight into the whole protein composition of SCs.ResultsTwo-dimensional liquid chromatography coupled with tandem mass spectrometry (2D LC-MS/MS) was performed to identify the protein expressions in primary cultured SCs of rats. We identified a total of 1,232 proteins, which were categorized into 20 functional classes. We also used quantitative real time RT-PCR and Western blot analysis to validate some of proteomics-identified proteins.ConclusionWe showed for the first time the proteome map of SCs. Our data could serve as a reference library to provide basic information for understanding SC biology.
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DOI：
10.1016/j.bbadis.2011.01.012
发表时间：
2011-05
影响因子：
6.2
作者：
Broadwater, Laurie;Pandit, Ashish;Clements, Robert;Azzam, Sausan;Vadnal, Jonathan;Sulak, Michael;Yong, V. Wee;Freeman, Ernest J.;Gregory, Roger B.;McDonough, Jennifer
通讯作者：
McDonough, Jennifer
影响因子：
3.3
作者：
Foeldi, Istvan;Mueller, Geza;Janaky, Tamas
通讯作者：
Janaky, Tamas
影响因子：
4.2
作者：
Gao, Wei-Min;Chadha, Mandeep S.;Kochanek, Patrick M.
通讯作者：
Kochanek, Patrick M.
影响因子：
3.4
作者：
Maurya, Devendra Kumar;Sundaram, Curam Sreenivasacharlu;Bhargava, Purnima
通讯作者：
Bhargava, Purnima
影响因子：
6.2
作者：
Mclaughlin, P;Zhou, Y;Zhang, J
通讯作者：
Zhang, J