Strain differences in early mouse embryo development in vitro: role of the nucleus.
Strain differences in early mouse embryo development in vitro: role of the nucleus.
复制标题
小鼠早期胚胎体外发育的品系差异：细胞核的作用。
DOI：
10.1002/jez.1402470308
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
N. First
中科院分区：
文献类型：
--
作者：
J. Robl;J. K. Lohse;N. First
许多品系的小鼠胚胎在单细胞阶段培养时，在两细胞阶段的发育受到阻碍。然而，如果将它们置于双细胞阶段培养，它们就会以很高的速率发育成囊胚。我们通过分析注射基因的蛋白质产物的酶活性来研究封闭胚胎中的转录和翻译系统，并发现这些系统功能正常。通过封闭胚胎和非封闭胚胎之间的相互核移植来研究细胞核的作用。结果表明，对于单细胞阶段培养的胚胎的成功发育，细胞核的来源与细胞质的来源同等重要。然而，当从双细胞阶段开始培养时，细胞核是造成培养率菌株差异的唯一原因。
Mouse embryos of many strains are blocked in development at the two-cell stage when placed in culture at the one-cell stage. If, however, they are placed in culture at the two-cell stage, they develop to blastocysts at a high rate. We investigated the transcriptional and translational systems in blocked embryos by assaying enzymatic activity of the protein product of an injected gene and found these systems to be functioning normally. The role of the nucleus was investigated by reciprocal nuclear transplants between blocked and nonblocked embryos. The results indicate that the source of the nucleus was of equal importance to that of the cytoplasm for the successful development of embryos cultured from the one-cell stage. When cultured from the two-cell stage, however, the nucleus was solely responsible for strain differences in culture rates.
影响因子：
56.9
作者：
MCGRATH, J;SOLTER, D
通讯作者：
SOLTER, D