Cytoadherence Properties of Plasmodium knowlesi-Infected Erythrocytes.
Cytoadherence Properties of Plasmodium knowlesi-Infected Erythrocytes.
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DOI：
10.3389/fmicb.2021.804417
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发表时间：
2021
影响因子：
5.2
通讯作者：
Rénia L
中科院分区：
文献类型：
--
作者：
Lee WC;Shahari S;Nguee SYT;Lau YL;Rénia L
诺氏疟原虫是可能致命的人畜共患疟疾的罪魁祸首。虽然恶性疟疾的严重病理与恶性疟原虫感染的红细胞(IRBC)的细胞黏附现象有关，但关于诺氏疟原虫-IRBC的细胞黏附特性的信息仍然很少。在这里，我们表征了感染实验室适应的诺氏P.nowlesi A1-H.1菌株的红细胞的细胞黏附特性。我们发现，晚期红细胞以一种依赖于人血清的方式形成花环，并且花环阻碍了红细胞的吞噬。IRBC对来自大脑(hCMEC/D3)、肺(HPMEC)和肾脏(HRGEC)的未暴露(未受刺激)的人内皮细胞株没有很大的粘附力。然而，用诺氏假单胞菌培养上清液“启动”后，HPMEC和HRGEC的IRBC-内皮细胞黏附率增加，而hCMEC/D3细胞的黏附率无明显变化。诺氏红假单胞菌经胰酶处理后，内皮细胞黏附和玫瑰花环现象均被消除。我们还发现，不同的受体参与了IRBC对不同类型内皮细胞的黏附。尽管恶性疟原虫-IRBC和诺氏疟原虫-IRBC都有一些共同的宿主受体，但受体上的糖结合物的可用性可能会影响恶性疟原虫-IRBC与这些受体的细胞黏附能力。
Plasmodium knowlesi is responsible for zoonotic malaria infections that are potentially fatal. While the severe pathology of falciparum malaria is associated with cytoadherence phenomena by Plasmodium falciparum-infected erythrocytes (IRBC), information regarding cytoadherence properties of P. knowlesi-IRBC remained scarce. Here, we characterized the cytoadherence properties of RBC infected with the laboratory-adapted P. knowlesi A1-H.1 strain. We found that late-stage IRBC formed rosettes in a human serum-dependent manner, and rosettes hampered IRBC phagocytosis. IRBC did not adhere much to unexposed (unstimulated) human endothelial cell lines derived from the brain (hCMEC/D3), lungs (HPMEC), and kidneys (HRGEC). However, after being “primed” with P. knowlesi culture supernatant, the IRBC-endothelial cytoadherence rate increased in HPMEC and HRGEC, but not in hCMEC/D3 cells. Both endothelial cytoadherence and rosetting phenomena were abrogated by treatment of P. knowlesi-IRBC with trypsin. We also found that different receptors were involved in IRBC cytoadherence to different types of endothelial cells. Although some of the host receptors were shared by both P. falciparum- and P. knowlesi-IRBC, the availability of glycoconjugates on the receptors might influence the capacity of P. knowlesi-IRBC to cytoadhere to these receptors.
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影响因子：
6.4
作者：
Carvalho, Bruna O.;Lopes, Stefanie C. P.;Costa, Fabio T. M.
通讯作者：
Costa, Fabio T. M.
影响因子：
3
作者：
Cox-Singh J;Hiu J;Lucas SB;Divis PC;Zulkarnaen M;Chandran P;Wong KT;Adem P;Zaki SR;Singh B;Krishna S
通讯作者：
Krishna S
影响因子：
3.1
作者：
Chin AZ;Maluda MCM;Jelip J;Jeffree MSB;Culleton R;Ahmed K
通讯作者：
Ahmed K
影响因子：
13.2
作者：
Barber BE;Grigg MJ;Piera KA;William T;Cooper DJ;Plewes K;Dondorp AM;Yeo TW;Anstey NM
通讯作者：
Anstey NM
影响因子：
3
作者：
Hempel, Casper;Wang, Christian William;Staalso, Trine
通讯作者：
Staalso, Trine