OsSGL, a Novel DUF1645 Domain-Containing Protein, Confers Enhanced Drought Tolerance in Transgenic Rice and Arabidopsis.
OsSGL, a Novel DUF1645 Domain-Containing Protein, Confers Enhanced Drought Tolerance in Transgenic Rice and Arabidopsis.
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OsSGL 是一种新型 DUF1645 结构域蛋白，可增强转基因水稻和拟南芥的耐旱性
DOI：
10.3389/fpls.2016.02001
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发表时间：
2016
影响因子：
5.6
通讯作者：
Xu G
中科院分区：
文献类型：
--
作者：
Cui Y;Wang M;Zhou H;Li M;Huang L;Yin X;Zhao G;Lin F;Xia X;Xu G
干旱是限制植物生长和作物产量的主要环境因素。基因工程是提高包括水稻在内的多种作物耐旱性的有效途径。相关基因的功能表征是确定此类改进的候选者时的先决条件。我们研究了水稻中一个新的含DUF1645结构域的蛋白OsSGL（Oryza sativa Stress tolerance and Grain Length）。OsSGL在多种逆境胁迫下表达上调，并定位于细胞核。过量表达和异源表达OsSGL的转基因植株分别显著提高了转基因水稻和拟南芥的耐旱性。在渗透胁迫下，过量表达的植株积累了较高水平的脯氨酸和可溶性糖，但较低的丙二醛（MDA）含量。我们的RNA测序数据表明，一些胁迫响应基因显着改变转基因水稻植株。我们出乎意料地观察到，那些过表达的水稻植株也有广泛的根系，这可能是由于生长素和细胞分裂素相关基因的转录水平改变。这些结果表明，OsSGL赋予增强的耐旱性的机制是由于胁迫响应基因的调节表达，更高的渗透调节物质积累和扩大的根系。
Drought is a major environmental factor that limits plant growth and crop productivity. Genetic engineering is an effective approach to improve drought tolerance in various crops, including rice (Oryza sativa). Functional characterization of relevant genes is a prerequisite when identifying candidates for such improvements. We investigated OsSGL (Oryza sativa Stress tolerance and Grain Length), a novel DUF1645 domain-containing protein from rice. OsSGL was up-regulated by multiple stresses and localized to the nucleus. Transgenic plants over-expressing or hetero-expressing OsSGL conferred significantly improved drought tolerance in transgenic rice and Arabidopsis thaliana, respectively. The overexpressing plants accumulated higher levels of proline and soluble sugars but lower malondialdehyde (MDA) contents under osmotic stress. Our RNA-sequencing data demonstrated that several stress-responsive genes were significantly altered in transgenic rice plants. We unexpectedly observed that those overexpressing rice plants also had extensive root systems, perhaps due to the altered transcript levels of auxin- and cytokinin-associated genes. These results suggest that the mechanism by which OsSGL confers enhanced drought tolerance is due to the modulated expression of stress-responsive genes, higher accumulations of osmolytes, and enlarged root systems.
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影响因子：
2.6
作者：
Kim MH;Sato S;Sasaki K;Saburi W;Matsui H;Imai R
通讯作者：
Imai R
干旱引起的旱稻生根模式和根茎分配的变化
影响因子：
5.8
作者：
Asch, F;Dingkuhn, M;Audebert, A
通讯作者：
Audebert, A
DOI：
10.1107/s1744309110001685
发表时间：
2010-10-01
影响因子：
0.9
作者：
Bateman, Alex;Coggill, Penny;Finn, Robert D.
通讯作者：
Finn, Robert D.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
7.4
作者：
Bischoff, Volker;Nita, Silvia;Scheible, Wolf-Ruediger
通讯作者：
Scheible, Wolf-Ruediger