Histomorphometric analysis of a cell-based model of cartilage repair.
Histomorphometric analysis of a cell-based model of cartilage repair.
复制标题
基于细胞的软骨修复模型的组织形态计量学分析。
作者：
Kimberly M Spangenberg;G. Peretti;C. Trahan;M. Randolph;L. Bonassar
本研究在一个已建立的关节软骨修复模型中检查了软骨细胞的形态。从年轻的绵羊收获关节软骨，并将其接种到失活的绵羊软骨片上。将接种的碎片成对堆叠并包裹在纤维蛋白胶中，然后皮下植入无胸腺小鼠的背部。6周后收获样品，并通过透射电子显微镜（TEM）或光学显微镜检查。透射电镜观察发现，与失活软骨直接贴壁的细胞延长，胞质增大，边缘皱褶。远离支架组织界面的细胞的TEM显示具有大核的圆形细胞，其看起来类似于正常软骨细胞。组织学标本的定量形态测定显示，细胞面积，相对量的细胞质，细胞纵横比，和相对核位移都较高的细胞与支架组织的界面附近，并随着距离界面。细胞形态的这些指数都与细胞-支架界面处支架的主动重塑一致。
This study examined the morphology of chondrocytes in an established model of articular cartilage repair. Articular cartilage was harvested from young sheep and seeded onto pieces of devitalized sheep cartilage. The seeded pieces were stacked in pairs and wrapped in fibrin glue, and then implanted subcutaneously in the dorsum of athymic mice. Samples were harvested after 6 weeks and examined by transmission electron microscopy (TEM) or by light microscopy. TEM revealed that the cells in direct apposition to the devitalized cartilage were elongated, with an enlarged cytoplasm, and a ruffled border. TEM of cells far from the interface with scaffold tissue revealed rounded cells with large nuclei that appeared similar to normal chondrocytes. Quantitative morphometry of histologic specimens revealed that cell area, relative amount of cytoplasm, cell aspect ratio, and relative nuclear displacement were all higher in cells near the interface with the scaffold tissue, and decreased with distance from the interface. These indices of cell morphology are all consistent with an active remodeling of the scaffold at the cell-scaffold interface.
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无牙骨硬化大鼠具有正常的维生素 D 结合蛋白-巨噬细胞激活因子 (DBP-MAF) 级联，并且软骨发育不良对集落刺激因子 1 (CSF-1) 和/或 DBP-MAF 治疗具有抵抗力。
影响因子：
4.1
作者：
Odgren,PR;Popoff,SN;Safadi,FF;MacKay,CA;Mason-Savas,A;Seifert,MF;MarksJr,SC
通讯作者：
MarksJr,SC
人毛细血管内皮细胞中基质金属蛋白酶 2 激活的粘附依赖性控制。
影响因子：
4
作者：
Yan,L;Moses,MA;Huang,S;Ingber,DE
通讯作者：
Ingber,DE
影响因子：
2.8
作者：
SAAMANEN, AM;TAMMI, M;HELMINEN, HJ
通讯作者：
HELMINEN, HJ
机械压缩改变软骨外植体中单个细胞周围的蛋白聚糖沉积和基质变形。
影响因子：
4
作者：
Quinn,TM;Grodzinsky,AJ;Buschmann,MD;Kim,YJ;Hunziker,EB
通讯作者：
Hunziker,EB
DOI：
10.2106/00004623-199304000-00009
发表时间：
1993-04-01
影响因子：
5.3
作者：
SHAPIRO, F;KOIDE, S;GLIMCHER, MJ
通讯作者：
GLIMCHER, MJ